Question

Consider cargo proteins that are destined for secretion to the extracellular space.

For each of the following pairs of mutations or conditions, predict where the cargo

will end up (ex. in the cytoplasm, a specific organelle, transport vesicles, multiple

places, extracellular space) for: (i) & (ii) Each individual condition; & (iii) The

combination of both.

(i) A mitochondrial signal sequence is added to the C-terminus of the cargo protein.

(ii) v-SNAREs on COPII coated vesicles are mutated to be unable to bind t-SNAREs

(iii) Both