Consider cargo proteins that are destined for secretion to the extracellular space. Please predict where the cargo will end up (ex. in the cytoplasm, a specific organelle, transport vesicles, multiple places, extracellular space) for: (i) & (ii) Each individual condition; & (iii) The combination of both conditions.
A.
(i) Normal Sar1 is mutated so it binds tightly to GDP and cannot exchange it for GTP. (This leads to Sar1*-GDP being the only form of the protein in the cell.)
(ii) COPII coat proteins are mutated so they bind both to active Sar1 and active ARF1.
(iii) Both
B. (i) A KDEL signal sequence is added to the C-terminus of the cargo protein.
(ii) The signal recognition particle is mutated so it canât recognize ER signal sequences.
(iii) Both
Consider cargo proteins that are destined for secretion to the extracellular space. Please predict where the cargo will end up (ex. in the cytoplasm, a specific organelle, transport vesicles, multiple places, extracellular space) for: (i) & (ii) Each individual condition; & (iii) The combination of both conditions.
A.
(i) Normal Sar1 is mutated so it binds tightly to GDP and cannot exchange it for GTP. (This leads to Sar1*-GDP being the only form of the protein in the cell.)
(ii) COPII coat proteins are mutated so they bind both to active Sar1 and active ARF1.
(iii) Both
B. (i) A KDEL signal sequence is added to the C-terminus of the cargo protein.
(ii) The signal recognition particle is mutated so it canât recognize ER signal sequences.
(iii) Both
