More often than not, the AMA will not approve a new, permanent CPT code in the US until all of the following conditions have been met except:

a. Pivotal trials have been performed and the results published

b. There is evidence of widespread adoption in the market

c. A health technology assessment has been performed, with favorable results

d. FDA approval/clearance has been granted

Read a "Global epistasis makes adaptation predictable despite sequence-level stochasticity" by Kryazhimskiyet al. (2014)

Do you believe the author’s data presented in the paper (and any supplements) support their hypothesis. If not, why not?

Are there other experiments or experimental modification that should have been performed by the researchers?

You performed a soil extraction (1 mL peptone solution for 1 g soil) as the extracting solution. A dilution and plating technique is performed using the spread plate method by spreading 0.1 mL of diluted solution in agar plates. The following results are obtained:

a. 150 colonies are counted on an R2A plate at the 105 dilution; b. 30 colonies are counted on a nutrient (nutrient rich) agar plate at 104 dilution.

Calculate the number of viable bacteria per gram of soil for R2A and nutrient agar. Why are the counts for nutrient agar lower than for the R2A media?