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11 Dec 2019

Describe the important anatomical differences among the three genera Bacillus, Escherichia and Mycobacteria and the staining protocols you would use to identify which genus is causing a patient's infection.

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Patrina Schowalter
Patrina SchowalterLv2
13 Dec 2019
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Related questions

These Questions Are in regards to Stainingtechniques.

Endospores

1) Under what conditions are endospores usually formed?

2) How do Bacillus and Clostridium genera differ in their oxygenpreference?

3)After Staining, why do endospores appear green but the rest ofthe cell pink?

4) Name 3 diseases caused by anaerobicendospore forming organisms and one caused byaerobic endospore forming organisms.

Acid Fast Stain

1) The mycobacteria appear fuchsia with an Acid Fast Stain andpurple with a Gram Stain. Explain his phenomenon.

2) Why is it desireable to combine S. aureus with acid fastorganisms such as M. smegmatis when applying the Acid Fast stainingtechnique?

3) Name two human diseases and their causative agents where theAcid Fast stain is used in diagnosis.

Would greatly appreciate as detailed of an answer aspossible.

How is DNA sequencing a helpful addition to gram staining and biochemical testing when identifying bacterial species?

Define the term “aseptic”; explain how this term could be used to describe both microbiology lab and patient care protocols

Describe the inoculation method, stain, and growth media choices you might use to isolate a pure culture in the laboratory

1. how important are capsules for bacteria to be able to cause disease?

2. why is it nessary to use a negative stain technique to view capsules? in you answer, be sure to include a definition of negative stain.

3. In preforming the spore stain, why is it necessay to heat malchite green?

4. In a natural habitat such as soil, what advantages does endospores production afford to members of the genera Bacillus and Clostridum over the nonsporing bacteria?

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