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These Questions Are in regards to Stainingtechniques.

Endospores

1) Under what conditions are endospores usually formed?

2) How do Bacillus and Clostridium genera differ in their oxygenpreference?

3)After Staining, why do endospores appear green but the rest ofthe cell pink?

4) Name 3 diseases caused by anaerobicendospore forming organisms and one caused byaerobic endospore forming organisms.

Acid Fast Stain

1) The mycobacteria appear fuchsia with an Acid Fast Stain andpurple with a Gram Stain. Explain his phenomenon.

2) Why is it desireable to combine S. aureus with acid fastorganisms such as M. smegmatis when applying the Acid Fast stainingtechnique?

3) Name two human diseases and their causative agents where theAcid Fast stain is used in diagnosis.

Would greatly appreciate as detailed of an answer aspossible.

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Deanna Hettinger
Deanna HettingerLv2
29 Sep 2019
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