Question

QUESTION 1

One major challenge for the eukaryotic transcription machinery not faced by its prokaryotic counterpart is

A) transcribing complex transcription units into polycistronic mRNA as compared to the simple, typically monocistronic transcription units of prokaryotes.

B) is dealing with the fact that eukaryotic DNA is packaged into neucleosomes and higher-order forms of chromatin structure, while prokaryotic DNA is not.

C) is the need to engage transcription activators and disengage transcription repressors.

D) is unwinding the DNA to allow RNA polyemerase to transcribe the template-strand.

QUESTION 2

Which is the first factor to bind at the promoter of eukaryotic genes?

A) TFIIA

B) TATA box-binding protein

C) RNA polymerase

D) TFIIB

E) TFIIH

QUESTION 3

The fidelity of RNA pol II refers to

A) the enzyme's ability to identify the correct promoter from which to initiate transcription.

B) the enzyme's ability to initiate transcription without the need for a preexisting primer.

C) the enzyme's ability to proceed along the DNA while sequentially catalyzing the addition of new NTPs to the growing RNA chain, without detaching prematurely from the DNA.

D) the enzyme's ability to select the correct nucleotide to add to the growing RNA chain.

QUESTION 4

Are the following components of transcription regulation in eukaryotes cis-acting elements or trans-acting factors? (a.cis-acting element or b.trans-acting factor)

A) TATA-box

B) general transcription factors

C) enhancer

D) initiator

E) mediator

F) TATA-binding protein