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19 Nov 2019

2) Which of the following seals the sticky ends of restriction fragments to make recombinant DNA?

A) restriction enzymes.

B) gene cloning.

C) DNA ligase.

D) gel electrophoresis.

E) reverse transcriptase.

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Related questions

How are restriction enzymes used to insert foreign DNA into plasmids?

a. use the same restrcition enzyme to cut the insert and the vector, then ligate them together with DNA ligase

b. use a restriction enzyme to cut the insert into several fragments, then allow the fragments to recombine with the vector

c. use a restriction enzyme to cut the vector into several fragments, then use DNA ligase to reassemble the fragments around the insert.

d. use a unique restriction enzyme to cut the insert and the vector, then use Taq polymerase to extend the sticky ends, followed by treatment with DNA ligase

e. none of these procedures will work

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What is recombinant DNA technology?What is a vector?What is found in a typical plasmid?How can DNA sequenced use the dideoxy chain termination method? Describe the polymerase chain reaction (PCR). What are some advantages and limitations of this procedure?What is a cDNA? How are cDNAs and DNA microarrays used to study gene expression?Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology,Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA moleculOutline the procedures for cloning a eukaryotic gene in a bacterial plasmid.Describe techniques that allow identification of recombinant cells that have taken up a gene of interest.How can you use the lacZ gene to facilitate cloning ?Distinguish between genomic and cDNA libraries.Define genomics and proteomics.Explain how gel electrophoresis is used to analyze nucleic acids and to distinguish between two alleles of a gene.How does dideoxy sequencing work? What is the significance of using dideoxy nucleotides?What are BACs, YACs, and contigs? What are some practical applications of DNA technology?What are the ways in which organisms can be genetically modified?

Restriction enzymes are used to construct restriction maps ofDNA. These are diagrams of specific DNA molecule that show thesites where the restriction enzymes cleave the DNA. To construct arestriction map, purified samples of the DNA are treated withrestriction enzymes, either alone or in combination, and then thereaction products are separated by agarose gel electrophoresis, atechnique that separates the DNA fragments based on size. (Thesmaller fragments travel more quickly and are found near the bottomof the gel, whereas the larger fragments are found near the top ofthe gel.)

Use the results of the agarose gel electrophoresis separation(above) to construct a restriction map for the sample of DNA.

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