cdaselb13044

1. During DNA replication, proteins unwind the DNA duplex, and separate the DNA strands. Those proteins include:

a.) DNA polymerase and ligase

b.) Single-stranded DNA binding and protein primase

c.) Helicase and DNA polymerase

d.) Helicase and single-stranded DNA binding protein

2. What determines the nucleotide sequence of the newly synthesized strand during DNA replication?

a.) The particular DNA polymerase catalyzing the reaction

b.) The relative amounts of the four nucleotide triphosphates (ATP, GTP, CTP, and TTP) in the cell

c.) The nucleotide sequence of the template strand

d.) The primase used in the reaction

e.) Both A and D

3. The leading and the lagging strands differ in that

a.) The leading strand is synthesized in the same direction as the movement of the replication fork, and the lagging strand is synthesized in the opposite direction.

b.) The leading strand is synthesized by adding nucleotides to the 3’ hydroxyl (OH) end of the growing strand, and the lagging strand is synthesized by adding nucleotides to the 5’ phosphate (PO4) end.

c.) The leading strand is synthesized continuously, whereas the lagging strand is synthesized in short fragments that are ultimately stitched together.

d.) Both A and B

e.) Both A and C

1. During DNA replication, proteins unwind the DNA duplex, and separate the DNA strands. Those proteins include:

a.) DNA polymerase and ligase

b.) Single-stranded DNA binding and protein primase

c.) Helicase and DNA polymerase

d.) Helicase and single-stranded DNA binding protein

2. What determines the nucleotide sequence of the newly synthesized strand during DNA replication?

a.) The particular DNA polymerase catalyzing the reaction

b.) The relative amounts of the four nucleotide triphosphates (ATP, GTP, CTP, and TTP) in the cell

c.) The nucleotide sequence of the template strand

d.) The primase used in the reaction

e.) Both A and D

3. The leading and the lagging strands differ in that

a.) The leading strand is synthesized in the same direction as the movement of the replication fork, and the lagging strand is synthesized in the opposite direction.

b.) The leading strand is synthesized by adding nucleotides to the 3’ hydroxyl (OH) end of the growing strand, and the lagging strand is synthesized by adding nucleotides to the 5’ phosphate (PO4) end.

c.) The leading strand is synthesized continuously, whereas the lagging strand is synthesized in short fragments that are ultimately stitched together.

d.) Both A and B

e.) Both A and C

What accounts for the difference in how the leading and lagging strands of DNA are synthesized? a. DNA polymerase can work on only one strand at a time. b. The origins of replication occur only at the 5' end. c. DNA polymerase can join new nucleotides only to the 3' end of a growing strand. d. DNA ligase works only in the 3' 5' direction.

1) What is the enzymatic function of restriction enzymes?

A) To add new nucleotides to the growing strand of DNA.

B) To join nucleotides during replication.

C) To join nucleotides during transcription.

D) To cleave nucleic acids at specific sites.

E) To repair breaks in sugar-phosphate backbones

1) What is the enzymatic function of restriction enzymes?

A) To add new nucleotides to the growing strand of DNA.

B) To join nucleotides during replication.

C) To join nucleotides during transcription.

D) To cleave nucleic acids at specific sites.

E) To repair breaks in sugar-phosphate backbones.

2) Which of the following seals the sticky ends of restriction fragments to make recombinant DNA?

A) restriction enzymes.

B) gene cloning.

C) DNA ligase.

D) gel electrophoresis.

E) reverse transcriptase.

2) Which of the following seals the sticky ends of restriction fragments to make recombinant DNA?

A) restriction enzymes.

B) gene cloning.

C) DNA ligase.

D) gel electrophoresis.

E) reverse transcriptase.

Which of the following reagents and biomolecules are necessary to make a recombinant DNA ?

a. Restriction enzyme e. Chymotrypsin

b. DNA fragment to be cloned f. Calcium chloride

c. Glucose g. starch

d. DNA ligase h. Ampicillin