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19 Apr 2019

If only a few percent of our genomic DNA ends up in exons... where is the majority of it?

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Related questions

(a) Do you expect more null mutations in a gene region with many large introns and a few small exons or a region of the same length with several large exons and only a few small introns? Why? (b) In what parts of an intron and what parts of an exon is a mutation more likely to cause a loss of function?

I've been reading a bit about "junk DNA" and how much of ourgenome consists of this "non coding DNA" in comparison to "codingDNA".

I'm just an interested layperson but I thought all combinationsof three base pairs encoded one amino acid, with some amino acidsbeing encoded by more than one combination of base pairs.

But if that were true then all of our DNA would encodesomething.

Or if only a tiny percentage of our DNA is "coding" that wouldmean that the vast majority of possible combinations of three basepairs don't represent any amino acid.

Or it could mean that there are a small number of "meaningless"combinations of three base pairs, but that those combinations arevastly overrepresented in our genome.

Which is correct? What am I missing?

1. The lungfish Protopterus aethiopicus has a genome 38 timeslarger than that of humans. Most of the DNA in this species isnoncoding repetitive DNA. What type of library would allow you tocompare just the genes in the lungfish to the genes in humans?

Select one:

a. Transgenic library

b. Genomic library

c. cDNA library

d. PCR library

e. Knockout library

2.Gel electrophoresis can be used to separate DNA on the basisof

Select one:

a. nucleotide content.

b. Both a and b

c. the probe used.

d. size.

e. electrical charge.

3.Which of the following is FALSE of DNA sequences within a cDNAlibrary?

Select one:

a. They do not contain promoters.

b. They do not contain exons.

c. They do not contain introns.

d. They do not contain enhancers.

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