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1.When does DNA replication occur in the life of a prokaryotic cell? When does it take place in the life of a eukaryotic cell? Is it always for the same purpose?

2.What proteins are necessary for DNA replication and what are their functions?

3.What is an origin of replication? How does it differ in prokaryotes and eukaryotes?

4.Why are primers needed and how are they made? What is lagging strand synthesis and why is it necessary?

5.What is the difference between a replication fork and a replication bubble?

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Keith Leannon
Keith LeannonLv2
28 Sep 2019
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Related questions

5. A) DnaB is a helicase that loads as a ring onto one strand ofthe DNA and pushes it apart from the second strand. This allows for5รขย€ย™-3รขย€ย™ DNA unwinding and replication fork advancement that isnecessary for initiation and elongation. If you create atemperature sensitive mutation in DnaB, what will be the effect onDNA replication (quick stop/slow stop mutation) at thenon-permissive temperature? Why does this occur?

B) DnaA is a protein that binds to OriC (origin of replication)to initiate DNA replication. This protein must be made new witheach round of replication. If you create a temperature sensitivemutation in DnaA, what will be the effect on DNA replication (quickstop/slow stop) at the non-permissive temperature? Why does thiseffect occur?

I'm currently reading a little about DNA replication, and havecome accross the following statement;

Replication starts from a fixed point and is bi-directional ...In Eukaryotes, there are multiple replication forks, eachprogressing in a bi-directional fashion.

If there is a single, long strand of DNA in a Eukaryotic cell, Isee potential problems with this:

These forks involve opening up a section of double-stranded DNA,and each strand becoming a double strand in a newly synthesisedpiece of DNA. At some point, before any single fork has become twonew double-stranded molecules, another fork could 'collide' withthis, causing it to attempt to replicate the non-finishedsection.

Simply, how can one replication fork meet another without eitherexponentially increasing the number of strands beingreplicated?

Also, on a more general level, I would be quite interested toknow the actual benefit of this, when, typically, only a singlecopy of the double-stranded molecule needs making.

QUESTION 1. Choose the true statement about prokaryotic and eukaryotic DNA replication.

A.Eukaryotic DNA replication involves less protein factors than does prokaryotic DNA replication.
B. Prokaryotic DNA polymerases replicate DNA 5' to 3', while eukaryotic DNA polymerases replicate DNA 3' to 5'.
C. Eukaryotic DNA replication begins at multiple origins of replication, while prokaryotic DNA replication begins at a single origin of replication.
D. Prokaryotic genomes take longer to replicate than eukaryotic genomes.

QUESTION 2. Bacterial cells use DNA replication to:

A. make back-up copies of their genome in case of a mutation.
B. manifest multiple phenotypes at once.
C. copy their genetic material prior to binary fission.
D. build functional proteins.

QUESTION 3. Choose the true statement about the genetic code.

A. A cell's mRNA code is read four nucleotides at a time to specify a single amino acid.
B. The genetic code encodes 64 amino acids, one for each of the 64 corresponding codons.
C. The genetic code is redundant, with multiple codons specifying a single amino acid.
D. The genetic code varies widely among organisms, with each having a different set of codons for a corresponding amino acid.

QUESTION 4. Of the following enzymes, which are correctly matched with their function? To be marked correct, you'll need to select all correctly matched statements, as there may be more than one correct answer.

A. Helicase, relieves torsion stress as DNA unwinds
B. Ligase, covalently bonds adjacent Okazaki fragments
C. Primase, removes RNA primers
D. DNA polymerase III, builds most of the DNA on the leading and lagging strand

QUESTION 5. Which of the following contributes to DNA replication accuracy? To be marked correct, you'll need to select all true statements, as there may be more than one correct answer.

A. The capacity to proofread improves DNA replication accuracy.
B. A slow rate of DNA replication contributes to accuracy.
C. The use of primase improves DNA replication accuracy.
D. DNA replication accuracy is improved thanks to complementary base pairing rules.

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