5. A) DnaB is a helicase that loads as a ring onto one strand ofthe DNA and pushes it apart from the second strand. This allows for5â-3â DNA unwinding and replication fork advancement that isnecessary for initiation and elongation. If you create atemperature sensitive mutation in DnaB, what will be the effect onDNA replication (quick stop/slow stop mutation) at thenon-permissive temperature? Why does this occur?
B) DnaA is a protein that binds to OriC (origin of replication)to initiate DNA replication. This protein must be made new witheach round of replication. If you create a temperature sensitivemutation in DnaA, what will be the effect on DNA replication (quickstop/slow stop) at the non-permissive temperature? Why does thiseffect occur?
5. A) DnaB is a helicase that loads as a ring onto one strand ofthe DNA and pushes it apart from the second strand. This allows for5â-3â DNA unwinding and replication fork advancement that isnecessary for initiation and elongation. If you create atemperature sensitive mutation in DnaB, what will be the effect onDNA replication (quick stop/slow stop mutation) at thenon-permissive temperature? Why does this occur?
B) DnaA is a protein that binds to OriC (origin of replication)to initiate DNA replication. This protein must be made new witheach round of replication. If you create a temperature sensitivemutation in DnaA, what will be the effect on DNA replication (quickstop/slow stop) at the non-permissive temperature? Why does thiseffect occur?
