BIO130H1 Lecture Notes - Lecture 12: Rna Polymerase Ii, Intron, Exon
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BIO130H1 Full Course Notes
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Document Summary
Phosphorylation of c-terminal tail of rna polymerase ii results in binding of various proteins e. g. 5" capping enzymes, spliceosomes, ctd, cleavage factors. Two step process: adenosine attacks the 5" splice site. 2)3" of one exon reacts with 5" of next exon to release water. The catalytic mechanism is rna dependent: oh of the ribose sugar is not present in deoxyribose, this group is necessary for the formation of the lariat structure in intron splicing. Transcription of the consensus sequences and recruitment of modifying proteins. Rna processing to generate the 3" end also required. Consensus sequences direct cleavage and polyadenylation of the 3" end. These sequences are encoded by the dna to be transcribed. Specific proteins recognize these sequences in the rna molecule. Cleavage and addition of a poly-a 3" tail result in the mature mrna. Transcription & rna processing putting it all together. Mature mrna exported from the nucleus to the cytoplasm. 1)translation in prokaryotes and eukaryotes including quality control.
