BIO130H1 Lecture Notes - Lecture 6: Base Pair, Eef-1, Cytosol
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BIO130H1 Full Course Notes
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Rna polymerase ii processing: (eukaryotes: firstly, exons and introns are present. Introns are spliced out and exons are present in final transcript. 5" cap added to mrna to protect it: cleavage of primary transcript, removal of 3" prime by nuclease and polyadenylation (adding lots of. Phosphorylation of c-terminal tail of rna polymerase ii results in binding of rna processing proteins. 5" capping enzymes adds 5" cap to mrna as soon as it is produced: spliceosome splices introns, cleavage/polyadenylation factors also attracted. Gmp: 5" to 5" linkage / triphosphate bridge, rna methyltransferase will methylate (add methyl group) the guanosines, sometimes ribose sugar will also be methylated in some caps, final step is binding of cap binding complex (cbc) a. If a mrna doesn"t have a 5" cap, there is something wrong: the small mrna strips created before the will be degraded due to lack of 5" cap. elongation mode.
