BIO130H1 Lecture Notes - Lecture 6: Aminoacyl-Trna, Alternative Splicing, C-Terminus
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BIO130H1 Full Course Notes
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Introns: uses alternative splicing, codes for multiple types of genes in a limited amount of space, it is a unique solution. *in eukarotes, a different rna is doing the attacking and cutting of phosphodiester bonds (snrnas) Splicing in dna: 2" oh group of the ribose sugar is not present in deoxyribose, and it is necessary for the lariat structure in the intron splicing, self-splicing dna could be a huge problem. Sequences: there are splice sites where the rna knows where to cut introns and exons, all sites are pretty consistent throughout all organisms, 15% of diseases are caused by bad splicing/mutations at the intron/exon boundary. Rna polymerase will continue binding unaware that the sequence is terminated. Dna polymerase, it has to recognize the termination. Leaving the nucleus: it is checked" to see if it is a 5" cap, without one, the mrna will degrade. Nuclear exosomes: 3" to 5" exonucleases that check for the proper mrna.
