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The following is the sequence of bases in the sense strand of a DNA segment and contains the beginning of a gene.

DNA 3? A T A T T A C C A G G C A T G G A C C C C C G G G 5?

Based on this sequence, write the sequence of the anti-sense DNA strand and the mRNA. Label the 5? and 3? ends in your predicted sequences. The start codon in this organism is AUG. Indicate where the start codon is in your sequence. Why is the start codon important? Why does there have to be a specific start codon?

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Patrina Schowalter
Patrina SchowalterLv2
28 Sep 2019

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Related questions

2) For the hypothetical DNA sequence/gene below mark the start codon and the stop codon of the gene. TSS->

5’CATAAA| GCAAACAGGAGATGGGCCCCAAAAGCCATGAATGGGAGCGCGTATAATCATGATTTATAATCTAGG TATCTCACTGA3’

3’GTATTT| CTTTTGTCCTCTACCCGGGGTTTTCGGTACTTACCCTCGCGCATATTAGTACTAAATATTAGATCC ATAGAGTGACT5’

Terminator sequence

Promoter sequence

TSS-> transcription start site and direction of transcription

a) Which strand of the DNA shown above, the top or the bottom, is the template strand?

b) What is the sequence of the mRNA produced from this gene? Label the 5’ and 3’ ends.

c) What is the sequence of the protein produced from the mRNA in (b)? Label the N and C termini.

d) Generate two examples each that result in a silent mutation, a missense mutation, a nonsense mutation, and a frameshift mutation for the DNA sequence above. Show the respective amino acids below each codon. (Courier font is a font typically used for sequences.)

Below is a DNA sequence that is a template strand and contains an Open reading frame for a gene:

3’ TAAACTGTACAGGGCCATAACT 5’

A. Find the open reading frame and circle the start and stop codon.

B. Transcribe the open reading frame into a strand of mRNA

C. What is the peptide encoded by this strand of mRNA?

D. Consider the 2nd translated codon from the mRNA strand. What is the probability that a single base change in any position of this codon would change the identity of the second amino acid in the protein sequence?

1) Explain how the Ames test can be used to detect potential mutagens? What are the limitations of this test?

2) The base pair sequence of a region of a gene coding for a polypeptide is:

Strand A: 5’ C A C C T A A C T T A C T C A G* C T T A C G A A G T C T A C C C A G C G 3’

Strand B: 3’ G T G G A T T G A A T G A G T C* G A A T G C T T C A G A T G G G T C G C 5’

A.) If this gene codes for a polypeptide that contains three Tyrosines (Tyr), which strand is transcribed? (1 pt)

B). What is the nucleotide of the mRNA transcribed from this DNA? (Note: there is no “start” codon. Simply start from the “appropriate” end). You MUST label the appropriate 5 ‘ and 3’ ends.

C.) What is the polypeptide sequence of the protein translated from this mRNA?

D.) Hydroxylamine (HA) affects the bold-faced (*) base pair and causes a mutation during DNA replication. What is the amino acid sequence of the polypeptide coded by the mutant gene?

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