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27 Feb 2018

Below is a DNA sequence that is a template strand and contains an Open reading frame for a gene:

3â TAAACTGTACAGGGCCATAACT 5â

A. Find the open reading frame and circle the start and stop codon.

B. Transcribe the open reading frame into a strand of mRNA

C. What is the peptide encoded by this strand of mRNA?

D. Consider the 2nd translated codon from the mRNA strand. What is the probability that a single base change in any position of this codon would change the identity of the second amino acid in the protein sequence?

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Casey DurganLv2

28 Feb 2018

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Related questions

A single strand of bacterial DNA contains the base sequence:

5â CGT GTA TTC ACA CTG GTG AGC CAC TAT CGT ATA TTC CCT GAG TGA GCA TTG 3â

A. What is the complementary sequence? Write this sequence below with its polarity.

B. Write the 6 reading frames below (group nucleotides in codon, i.e. ACGCVGTG â¦ = ACG CVG TG. ...) ?

C. Is there an open reading frame (ORF) at least 4 codons long? If yes, what is the reading frame (+1,+2,+3, -1,-2 or -3) for the ORF?

D. Does the strand of the DNA given in (a) serve as the coding strand or template strand for the synthesis of the RNA transcript?

Can someone please check and help me with these? Especially II and III?

I. Below is a DNA sequence that is a template strand and contains an Open reading frame for a gene:

3â TAA ATA CGT GAG GCA CTT CAA AGA CCG CAC ACT_ AAT TT 5â

5â AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA _ UUA AA 3â

a. Transcribe the DNA template into a strand of mRNA by typing the mRNA sequence below the template strand. Label the 5â and 3â end of the mRNA 5â AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA _ UUA AA 3â

b. Paste the mRNA below and do all 3 reading frames. Highlight all start codons green in all the reading frames. Highlight red the stop codons which are in frame with the start codons in all the 3 reading frames if they are present.

1) 5â AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA UUA AA 3â

2) 5â A UUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A 3â

3) 5â AU UUA UGC ACU CCG UGA AGU UUC UGG CGU GUG AUU AAA 3â

c) In which reading frame is the ORF found? The second one. Since it has AUG.

d) Paste the mRNA showing the most likely ORF below and translate the codons. Label the 5â and 3â end of the mRNA and the N- and C-termini of the peptide.

5â A UUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A 3â

MET HIS SER VAL LYS PHE LEU ARG CYS ASP STOP

N Terminus C Terminus

II. 3 pts. The base calling program made a mistake that you didnât catch and deleted the bolded G in the original template strand above. Paste the template DNA below and delete the G. Transcribe (show the codons) and translate the resulting sequence. Label the 5â and 3â ends of the DNA and mRNA and the N and C-termini of the peptide. Comment on the resulting peptide sequence.

5â AUUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A â3

MET HIS SER VAL LYS PHE LEU ARG CYS ASP STOP

III. 3 pts. The base calling program made a mistake that you didnât catch and added an extra G after the bolded G in the original template strand above. Paste the template DNA below and insert the extra G. Transcribe (show the codons) and translate the resulting sequence. Label the 5â and 3â ends of the DNA and mRNA and the N and C-termini of the peptide. Comment on the resulting peptide sequence.

5â AUUU AUG CAC UCC GUG AAG UUU CUG GGG UGU GAU UAA A â3

MET HIS SER VAL LYS PHE LEU GLY CYS ASP STOP

Which of the following is the best characterization of the reading frame in gene expression?

Question 5 options:

The reading frame of a gene is set by the first three nucleotides in a gene's promoter.

Every gene has three possible reading frames, but all three specify the same protein.

Every gene has three possible reading frames, and thus encodes three different proteins.

The reading frame of a gene is set by the first three nucleotides at the 5' end of the mRNA.

The reading frame of a gene is set by the first AUG codon near the 5' end of the mRNA.

Which of the following is/are NOT involved in initiating transcription in eukaryotes?

Question 9 options:

transcription factors

start codon

promoter

template strand of DNA

RNA polymerase

The chemical piece of DNA monomers that makes one DNA monomer different from another is:

Question 10 options:

	the sugar
	the position of the hydroxyl group on deoxyribose
	the nitrogenous base
	the position of the bond between phosphate and sugar
	the phosphate group

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