BIO-1201 Chapter Notes - Chapter 12: Dna Replication, Proteome, Noncoding Dna
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Be(cid:272)ause the ge(cid:374)eti(cid:272) (cid:272)ode is (cid:374)early u(cid:374)iversal, it"s possi(cid:271)le to tra(cid:374)sfer (cid:272)lo(cid:374)ed ge(cid:374)es between virtually any organism. To make rdna, researchers need a vector. Vector a piece of dna that acts as a carrier for the foreign dna: a common one is plasmid found in bacteria. Two enzymes needed to introduce foreign dna into plasmid dna: restriction enzymes cut dna, dna ligase-seal. A polymerase chain reaction creates billions of copies of a segment of dna in a test tube in a matter of hours: amplifies a targeted dna sequence. Pcr cycles over and over again doubling the amount of dna in each cycle. Pcr assists in dna fingerprinting by amplifying it: makes use of repeating noncoding dna segment, people differ in how many repeats. Electrophoresis separates samples by size: the longer the dna strands, the larger they are and the less they migrate. Reproductive cloning: desired end is an individual exactly like the original individual.
