BSC 1010C Lecture Notes - Lecture 14: Molecular Cloning, Genetically Modified Organism, Thermus Aquaticus

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1 Oct 2016
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Cloning is the production of identical copies of dna through some asexual means. An underground stem or root sends up new shoots that are clones of the parent plant. Members of a bacterial colony on a petri dish are clones because they all came from division of the same. Human identical twins are clones; the original single embryo separate to become two individuals. Gene cloning is production of many identical copies of the same gene. If the inserted gene is replicated and expressed, we can recover the cloned gene or protein product. Cloned genes have many research purposes: determining the base sequence between normal and mutated genes, altering the phenotype, obtaining the protein coded by a specific gene, etc. d. Humans can be treated with gene therapy: alteration of the phenotype in a beneficial way. Otherwise transgenic organisms are used to produce products desired by humans. To clone dna, scientists use recombinant dna (rdna) and polymerase chain reaction (pcr).

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