1. Consider the annexin protein family of peripheral proteins that require calcium to interact with the membrane. What residues
are likely to be on the surface that interacts membrane? Why?

What kinds of forces or bonds anchor an integral membrane protein in a biological membrane? What forces hold a peripheral membrane protein to the membrane?

It was discovered that a fraction of membrane proteins can be removed by immersing a membrane in an aqueous salt solution, whereas the remaining fraction of proteins can only be removed by immersing the membrane in a detergen solution or organic solvent which dissolves lipids.

Which fraction contains the peripheral proteins and which fraction contains the integral proteins and why? What does this experiment say about the way peripheral and integral proteins are bound to membranes?

Explain the difference between intergral membrane proteins,peripheral membrane proteins, and lipid-anchored membraneproteins