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Briefly explain the roles that nonhistone proteins play when associated with chromosomes. Why do chromosomes have more different types of nonhistone proteins than histone proteins?

The human genome contains about 3 billion base pairs. During the first cell division after fertilization of a human embryo, S phase is approximately 3 hrs long. Assuming an average DNA polymerase rate of 40 nucleotides/second, what is the minimum number of origins of replication you would expect to find in the human genome?

List 3 examples of types of mutations that would disrupt the process of mitotic chromosome segregation. How could you use yeast artificial chromosomes (YACs) to find these types of mutations in S. cerevisiae?

What DNA sequences are found at the telomeres of human chromosomes? Briefly explain the function of the two telomere-associated complexes (telomerase and shelterin).

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