1. Explain the principle of The Sanger method of DNA sequencing (40 marks)
2. Evaluate the original method compared to three alternative methods that have been developed(60 marks).

. Next Generation DNA Sequencing methods have dominated the study of microbial diversity in the last 12 years. Compare and contrast the Sanger vs the Illumina approach to DNA sequencing. First explain how each approach works, first the Sanger method then Illumina. Then, compare those two methods based on various Explain the principles of NGS its advantages and disadvantages as a tool for the characterization of microbial diversity.

Explain the workflow of sequencing a protein using the Sanger method or Edman degradation?

Compare and contrast older methods of protein sequencing with the current method of Mass Spectrometry?

How does dye-terminator cycle sequencing of DNA differ from conventional DNA sequencing procedures based on the original Sanger method?