Evaluate the use of blotting techniques and genotyping methods to detect gene expression in research settings

What are the differences and similarities of western blotting and northern blotting? (check all that apply) A) Both techniques require the separation of molecules to be detected by gel-electrophoresis first B) Western blotting is a technique that is commonly used to detect a protein of interest, while northern blotting is a technique that is commonly used to detect a mRNA of interest C) In western blotting, the proteins are separated horizontally, while in northern blotting, the proteins are separated vertically D) Western blotting allows the comparison of relative abundance of a protein of interest in different samples, while northern blotting allows detection of a protein of interest but not quantification E) Both techniques rely on a specific probe. In western blotting, specific antibody is used as a probe to detect a protein of interest, while in northern blotting, a specific oligo-nucleotides is used as a probe to detect a mRNA of interest

1) Northern blotting, RT-PCR, and microarrays can be used to analyze gene expression. A lab studies yeast cells, comparing their growth in two different sugars, glucose and galactose. One student is comparing expression of the gene HMG2 under these two conditions. Which technique(s) could he use and why? Another student wants to compare expression of all the genes on chromosome 4, of which there are approximately 800. What technique could she use and why?