What are the differences and similarities of western blotting and northern blotting? (check all that apply) A) Both techniques require the separation of molecules to be detected by gel-electrophoresis first B) Western blotting is a technique that is commonly used to detect a protein of interest, while northern blotting is a technique that is commonly used to detect a mRNA of interest C) In western blotting, the proteins are separated horizontally, while in northern blotting, the proteins are separated vertically D) Western blotting allows the comparison of relative abundance of a protein of interest in different samples, while northern blotting allows detection of a protein of interest but not quantification E) Both techniques rely on a specific probe. In western blotting, specific antibody is used as a probe to detect a protein of interest, while in northern blotting, a specific oligo-nucleotides is used as a probe to detect a mRNA of interest
What are the differences and similarities of western blotting and northern blotting? (check all that apply) A) Both techniques require the separation of molecules to be detected by gel-electrophoresis first B) Western blotting is a technique that is commonly used to detect a protein of interest, while northern blotting is a technique that is commonly used to detect a mRNA of interest C) In western blotting, the proteins are separated horizontally, while in northern blotting, the proteins are separated vertically D) Western blotting allows the comparison of relative abundance of a protein of interest in different samples, while northern blotting allows detection of a protein of interest but not quantification E) Both techniques rely on a specific probe. In western blotting, specific antibody is used as a probe to detect a protein of interest, while in northern blotting, a specific oligo-nucleotides is used as a probe to detect a mRNA of interest
Related textbook solutions
Related questions
Which of the following did Erwin Chargaff observe?
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The melting temperature (Tm) of a DNA duplex is:
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Given your knowledge and the descriptions of each of thesemethods, which of the following does NOT rely on the ability ofnucleic acids to hybridize with each other?
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Which of the following is a possible reason why DNA uses thymineinstead of uracil?
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Which of the following is an enzyme used in cloning to breakcovalent bonds?
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Which of the following could you use to remove the 5' phosphatesafter cleavage of a plasmid with a single type of restrictionenzyme to ensure that the plasmid would not simply fuse backtogether upon addition of ligase?
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Which of the following would you use to create a cDNA librarythat you would not use for a genomic library?
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A plasmid vector typically has which of the followingfeatures?
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Which of the following is FALSE of genomic or cDNAlibraries?
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Which of the following is NOT a typical component of apolymerase chain reaction (PCR)?
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How can a fusion protein be formed?
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Why is green fluorescent protein (GFP) so useful in visualizingfusion proteins in eukaryotes?
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You wish to determine the cellular location of a protein butunfortunately it is not particularly antigenic (can t develop astrong antibody response). How could you solve this problem?
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A positive result for the yeast two-hybrid analysis means thefollowing:
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Which of the following is the approximate size of the humangenome?
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Which of the following is correct about the structure orlocation of genes?
Question 22 options:
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