BIO314H5 Chapter Notes -Longfin Inshore Squid, Western Blot, Antiserum
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Primary antibody, mouse anti-myosin light chain antibody, were added. This was followed by 20 minutes of incubation on the rocking platform. The blocking solution was removed and 15ml of. Introduction the tbs-t wash buffer was added followed by. The purpose of this experiment was to isolate the myosin light chain protein using. The objectives of this experiment were to learn the techniques of protein extraction, immunolocalization to detect the exact location of the protein on the sds-page gel. Sds-page electrophoresis, coomassie staining, blotting and immunolocalization by analyzing hypothesis was that the immunoblot would detect either two or three bands per experimental unknown fish tissue samples. In essence, this lab served as an introduction to the field of lane for the fish tissues and that the squid tissue would show a noticeably different staining proteomics and the techniques used in it. The particular protein of interest for this experiment pattern on both the coomassie gel and the immunoblot.
