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5’ T C G C AA T A T G A A T A C G A G C A T T A T A G A C T T G T G 3’

3’ A G C G T T A T A C T T A T G C T C G T A A T A T C T G A A C A C 5’

Identify the -10 site for this prokaryotic sequence and the +1 site in order to determine what the resulting transcript will be from this sequence.

For your answer, write it in 5' to 3' orientation and write ONLY the letters (do not include the 5' and 5' or any dashes or spaces).

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Jamar FerryLv2
27 Dec 2021
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Related questions

DNA Structure and Function Lab Report

Summary Questions

  1. Name the three major components of DNA.

  1. Which nitrogen bases pair within DNA?

  1. What is the difference between replication, transcription, andtranslation?
  1. Describe an advantage of genetic engineering.

  1. List three differences between DNA & RNA.

DNA

RNA
  1. If the base sequence on one DNA strand is 3’ CCTAGTTAG 5’, whatwill the sequence be on the other strand of the helix duringreplication?

DNA

3’

C

C

T

A

G

T

T

A

G

5’

DNA

5’

3’

  1. If 3’ TGACCCATC 5’ serves as the template for transcription,what will be the sequence of nitrogen bases on the newly formed RNAstrand?

DNA

3’

T

G

A

C

C

C

A

T

C

5’

mRNA

5’

3’
  1. If a mRNA sequence is 3’ UAA 5’, give the amino acid coded forduring translation. Refer to the chart in Exercise 5 of theLab.

1. (20pts) Let’s consider how restrictive enzymes work. Look the two samples of DNA shown below:

DNA Sample #1

5’– T C C A G T G A T C T C G A A T T C G C T A G T A A C G T T C G A T

3’– A G G T C A C T A G A G C T T A A G C G A T C A T T G C A A G C T A

DNA Sample #2

5’– T C A T C G A A T T C C T G G A A T C A G C A G A A T T C G C A T A

3’– A G T A G C T T A A G G A C C T T A G T C G T C T T A A G C G T A T

If both samples are treated with the restriction enzyme EcoRI [recognition sequence G’AATTC] then indicate the number of fragments and the size of each fragment from each sample of DNA. List the fragment sizes in order of largest to smallest.

Sample # 1 Sample # 2

# of fragments:________ # of fragments:________

Sizes of fragments (largeà small): Sizes of fragments (largeà small):

(20pts) Read the protocol for Lab 8B.

What is supercoiled DNA

Why would it migrate further into an agarose gel if it’s uncut plasmid DNA?

What causes this type of DNA to form?

Is this likely to occur to our isolated plasmid DNA?

Based on the values given in discussion question 3.21 of the text, estimate the standard Gibbs energy, enthalpy and entropy changes involved in the formation of a hairpin loop of the following sequence of DNA.

5’-CCCACCGTGAGTCATCGCTAGACTCACGGTAAA-3’

Here are the values given in discussion question 3.21 of the text:

Sequence 5’-A-G 5’-G-C 5’-T-G

3’-T-C 3’-C-G 3’-A-C

ΔseqGΘ/(KJ mol-1) -5.4 -10.5 -6.7

ΔseqHΘ/(KJ mol-1) -25.5 -46.4 -31.0

ΔseqSΘ/(KJ mol-1) -67.4 -118.8 -80.8

This is for an upper division physical biochemistry class. please show all work. will rate thank you

You MUST use the given values or you will not recieve a positive rating. Thank you

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