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What results will be observed in Kligler's agar medium for a bacterium that can ferment glucose and lactose? What results will be observed for a bacterium that can ferment glucose but not lactose? Briefly explain the biochemical reason for the difference between these two results.

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Casey Durgan
Casey DurganLv2
13 Dec 2019
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Related questions

An organism can ferment lactose, and no gas is produced. What would you expect to see if you used this organism to inoculate glucose broth medium?

The broth will stay red and no bubble will be seen in the Durham tube.
The broth will stay red and a bubble will be seen in the Durham tube.
The broth will turn yellow and no bubble will be seen in the Durham tube.
The broth will turn yellow and a bubble will be seen in the Durham tube.

Q1. Will the omission of the 7.5% NaCl in MSA medium affect its specificity or sensitivity? Explain.

Q2. Will the addition of only 1/10th of mannitol in the MSA medium affect its specificity or sensitivity? Explain.

Q3. In clinical settings, Blood Agar plates are used to detect b-hemolysin producers. Suppose that after processing a patient’s sample, you saw growth on Blood Agar plate, but with no single colonies. Of the areas that showed growth, you observe full clearing of the blood cells. What conclusions would you draw about the presence of the b-hemolysin producers and a-hemolysin producers? Explain.

Q4. On EMB plates, colonies that would normally appear pink may turn into a metallic green shade if left sitting on the plate for multiple days. Explain why that is the case. Is this a specificity or sensitivity problem?

Q5. One of the features that separate E. coli from closely-related species are its ability to ferment lactose very efficiently. (A) If the lactose in the EMB plate is replaced with glucose, will it affect the specificity or sensitivity of the media?

(B) If the amount of lactose in EMB is reduced by 75%, will that impact the specificity or sensitivity of the medium?

18) In your research on gene regulation in E. coli, you produce 2 mutant strains with the same phenotype: they can grow on lactose but not on glucose. To investigate these 2 mutants further, you perform a complementation test using them and discover that the diploid combination strain can grow on glucose or lactose. From this, you can deduce that:

a.)Both mutants have a mutation in the same gene

b.)The 2 mutants have mutations in different genes
c.)Glucose and lactose metabolic pathways in E. coli utilize at least one shared gene in common
d.)At least 2 different genes are required for glucose metabolism in E. coli
e.) b) and d)

19. In your research on the lac operon, you produce a unique regulatory mutatant strain of E. Coli where the lac l gene's repressor protein binding site for the operator is normal (wild-type) but its lactose binding site no longer binds lactose. The expected phenotype of this mutant will be:

a. inability to grow on lactose only medium

b. ability to grow on lactose only medium

c. blue colony color when grown on glycerol plus x-gal medium

d. blue colony oclor when grown on lactose plus x-gal medium

e. b & d

20.

20) In your research on the lac operon, you create a diploid E.coli strain with the following genotype (+) = wild type gene, (-) = mutant gene.

I- /O+ /Z- /Y+

I+ /Oc /Z+ /Y+
Can this diploid mutant grow on medium containing lactose as the only carbon source?

a) Yes

b) No

c) Maybe, it depends on if the Oc mutation is dominant or recessive

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