4) Which of the following examples of how the process of eukaryotic transcription differs from the process of bacterial transcription is/are true?
A- Both eukaryotic and prokaryotic RNA polymerase can initiate transcription with the help of sigma factor where no additional proteins are needed
B- Bacterial cells contain a single RNA Polymerase known as mRNA, whereas eukaryotic cells have three RNA polymerases known as mRNA, tRNA and rRNA.
C- In eukaryotic cells, transcription regulators can influence transcriptional initiation thousands of nucleotides away from the promoter, whereas bacterial regulatory sequences are very close to the promoter.
D- Prokaryotic transcription is affected by chromatin structure and nucleosomes, whereas Eukaryotic are not.
E- All of the above are correct
F- Only A and B correct
G- Only B and C correct
H- None of the above is correct
6) You have a segment of DNA from bacteria that contains the following sequence (Check images file HW7 Q6 figure). Which of the following choices best describes how transcription occurs if you were told that the top strand is the template strand?
A. The RNA polymerase moves along the top strand from 5 to 3.
B. The RNA polymerase moves along the bottom strand from 3 to 5.
C. The RNA polymerase moves along the bottom strand from 5 to 3.
D. The RNA polymerase moves along the top strand from 3 to 5.
E. The RNA polymerase moves along the newly synthesized strand from 3 to 5.
F. Information is not enough to make a conclusion.
7) Using the same DNA sequence in question #6 above, write the down the RNA transcript if you learned that the top strand was your genic strand, assuming that the promotor sequence is in place and that the first nucleotide represent the start of the coding sequence. (clearly
label the 5 and 3 end following the same format in the figure. DO NOT INCLUDE ANY SPACE at the beginning or at the end of your answer, or between nucleotides. Do NOT add any comma)
9) Transcription in bacteria differs from transcription in a eukaryotic cell because...
A. RNA polymerase (along with its sigma subunit) requires the general transcription factors to assemble at the promoter before polymerase can begin transcription.
B. RNA polymerase does not need to be phosphorylated at its C-terminal tail for transcription to proceed.
C. RNA polymerase (along with its sigma subunit) can not initiate transcription on its own.
D. the sigma subunit must associate with the appropriate type of RNA polymerase to produce mRNAs.
10) Total nucleic acids are extracted from a culture of yeast cells and are then mixed with resin beads to which the polynucleotide 5-TTTTTTTTTTTTTTTTTTTTTTTT-3 has been
covalently attached. After a short incubation, the beads are then extracted from the mixture. When you analyze the cellular nucleic acids that have stuck to the beads, which of the following is most abundant?
DNA
rRNA
tRNA
mRNA
4) Which of the following examples of how the process of eukaryotic transcription differs from the process of bacterial transcription is/are true?
| A- Both eukaryotic and prokaryotic RNA polymerase can initiate transcription with the help of sigma factor where no additional proteins are needed |
| B- Bacterial cells contain a single RNA Polymerase known as mRNA, whereas eukaryotic cells have three RNA polymerases known as mRNA, tRNA and rRNA. |
| C- In eukaryotic cells, transcription regulators can influence transcriptional initiation thousands of nucleotides away from the promoter, whereas bacterial regulatory sequences are very close to the promoter. |
| D- Prokaryotic transcription is affected by chromatin structure and nucleosomes, whereas Eukaryotic are not. |
| E- All of the above are correct |
| F- Only A and B correct |
| G- Only B and C correct |
| H- None of the above is correct |
6) You have a segment of DNA from bacteria that contains the following sequence (Check images file HW7 Q6 figure). Which of the following choices best describes how transcription occurs if you were told that the top strand is the template strand?
| A. The RNA polymerase moves along the top strand from 5 to 3. |
| B. The RNA polymerase moves along the bottom strand from 3 to 5. |
| C. The RNA polymerase moves along the bottom strand from 5 to 3. |
| D. The RNA polymerase moves along the top strand from 3 to 5. |
| E. The RNA polymerase moves along the newly synthesized strand from 3 to 5. |
| F. Information is not enough to make a conclusion. |
7) Using the same DNA sequence in question #6 above, write the down the RNA transcript if you learned that the top strand was your genic strand, assuming that the promotor sequence is in place and that the first nucleotide represent the start of the coding sequence. (clearly
label the 5 and 3 end following the same format in the figure. DO NOT INCLUDE ANY SPACE at the beginning or at the end of your answer, or between nucleotides. Do NOT add any comma)
9) Transcription in bacteria differs from transcription in a eukaryotic cell because...
| A. RNA polymerase (along with its sigma subunit) requires the general transcription factors to assemble at the promoter before polymerase can begin transcription. |
| B. RNA polymerase does not need to be phosphorylated at its C-terminal tail for transcription to proceed. |
| C. RNA polymerase (along with its sigma subunit) can not initiate transcription on its own. |
| D. the sigma subunit must associate with the appropriate type of RNA polymerase to produce mRNAs. |
10) Total nucleic acids are extracted from a culture of yeast cells and are then mixed with resin beads to which the polynucleotide 5-TTTTTTTTTTTTTTTTTTTTTTTT-3 has been
covalently attached. After a short incubation, the beads are then extracted from the mixture. When you analyze the cellular nucleic acids that have stuck to the beads, which of the following is most abundant?
| DNA |
| rRNA |
| tRNA |
| mRNA |
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