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13 Feb 2018

1)Which of the following is NOT correct when considering the initiation of transcription in E. coli?

A) The Ï subunit of RNA polymerase makes strong contacts with the -10 region of promoters.

B) Only the -10 region of the promoter is required.

C) DNA melting near the transcription start site is essential for promoter recognition.

D) The phosphodiester bonds that are formed are not stable until about 10 nucleotides have been incorporated.

E) The RNA polymerase contacts about 50 bp of DNA.

2)In bacteria, mRNA, tRNA and rRNA are all transcribed by the same RNA polymerase.(TRUE/FALSE)

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Nestor RutherfordLv2

13 Feb 2018

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Related questions

Eukaryotic transcription normally takes place on a chromatin template at nuclease-accessible sites.(True/False)

*After promoter clearance by RNA polymerase II:

A) all associated factors remain bound to the polymerase for elongation.

B) a subset of factors remain at the promoter region.

*Which of the following statements is FALSE?

A) RNA polymerase II is responsible for the transcription of genes encoding protein-coding sequences only.

B) Many promoters recognized by RNA polymerase II contain regulatory elements several kbp upstream of the initiation site.

C) TFIIB has a role in converting a closed-promoter into an open-promoter complex.

D) The C-terminal domain of the largest subunit of RNA pol II undergoes extensive phosphorylation prior to transcription elongation.

E) A multiprotein complex is involved in communication between upstream control elements and proteins bound at the promoter.

C) no further elongation factors are acquired by the polymerase.

D) no further chromatin remodeling is required.

E) transcription continues until the polymerase reaches the poly (A) tail

*In eukaryotes RNA polymerase I transcribes all of the ribosomal RNA components.(TRUE/FALSE)

Which type of RNA is unique to eukaryotes?

		mRNA
		rRNA
		tRNA
		snRNA
		Its a trick, all cells have all four types of RNA.

1 points

QUESTION 2

The region of DNA to which RNA polymerase binds to initiate transcription is the:

		origin
		promoter
		enhancer
		none of the above are correct

1 points

QUESTION 3

Holoenzyme:

		is RNA polymerase core enzyme.
		is sigma factor.
		is core plus sigma factor.
		none of the above are correct.

1 points

QUESTION 4

Which of these is formed FIRST when RNA polymerase binds to a promoter?

		The closed promoter complex
		The open promoter complex
		A rho-independent terminator
		none of the above are correct.

1 points

QUESTION 5

A hairpin loop followed by a run of Us:

		is the sequence formed in the sense strand of a gene using a rho-independent terminator.
		is the sequence recognized by the rho protein.
		is the structure formed in RNA that causes rho-independent termination.
		is formed immediately after the open promoter complex is formed.

1 points

QUESTION 6

RNA polymerase:

		reads the template strand of the DNA in the 3' to 5' direction.
		synthesizes the RNA in the 5' to 3' direction.
		can begin transcription without a primer.
		all of the above are correct.

1 points

QUESTION 7

Which of the following statements is FALSE?

		Bacteria like E. coli have only one type of sigma factor.
		RNA polymerase has no proofreading activity.
		The promoter of a gene is upstream of the RNA-coding region.
		Bacterial transcriptional terminators are copied into RNA to cause termination.

1 points

QUESTION 8

If the template strand of a gene has the sequence 5' ATGCGAT 3', then:

		the RNA produced will have the sequence 5' AUGCGAU 3'.
		the RNA produced will have the sequence 5' UAGCGUA 3'.
		the RNA produced will have the sequence 5' AUCGCAU 3'.
		None of the above are correct.

1 points

QUESTION 9

The sense strand:

		is the lagging strand
		is also the template strand.
		always goes left to right.
		none of the above are correct.

1 points

QUESTION 10

Which of these is transcribed but not translated?

		mRNA
		tRNA
		rRNA
		all of the above
		tRNA and rRNA are transcribed but not translated.

please help i am a bit confused

Please provide brief (one sentence) answers:

a) The Mycoplasma chromosome codes for 500 genes, yet it has about 20 promoters. Provide a brief explanation for how this is possible.

b) How many seconds would it take the bacterial RNA polymerase to synthesize an mRNA of 5,000 bases?

c) How many mistakes would the bacterial RNA polymerase make while synthesizing an mRNA of 30,000 bases?

d) Sigma (?) protein by itself does not bind to promoter sites. Predict the effect on transcription of a mutation enabling ? to bind tightly to the â35 region in the absence of other subunits of RNA polymerase.

e) What is the structure of the most common transcription terminator signal in E. coli, and what are 2 conserved features of the RNA sequence of this structure?

f) Why is RNA synthesis not as carefully monitored for errors, as is DNA synthesis?