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18 Feb 2018
Why is such a seemingly complicated scheme used for LacZ complementation (X-gal colour selection and α-peptide complementation), in which most of the lacZ gene is in the host chromosome and the small α-peptide resides in the cloning vector? In other words, why not have the entire lacZ gene in the vector? Give two reasons. [3 marks] [Clue: From the relevant lectures and the Lab Manual, based on colour selection (Lecture 4) and the sizes of recombinants (Lecture 5)]
Why is such a seemingly complicated scheme used for LacZ complementation (X-gal colour selection and α-peptide complementation), in which most of the lacZ gene is in the host chromosome and the small α-peptide resides in the cloning vector? In other words, why not have the entire lacZ gene in the vector? Give two reasons. [3 marks] [Clue: From the relevant lectures and the Lab Manual, based on colour selection (Lecture 4) and the sizes of recombinants (Lecture 5)]
Nestor RutherfordLv2
18 Feb 2018
