BIO314H5 Study Guide - Midterm Guide: Plasmid, Real-Time Polymerase Chain Reaction, Nested Polymerase Chain Reaction
Document Summary
Plasmids naturally occurring genetic feature of bacteria. Has the ability to metabolize different antibiotics. Eliminate non-essential sequences: to reduced size to accommodate donor dna inserts. We can go ahead and grow bacteria to amplify dna: ori can be changed depending on the organism. Insert multiple restriction sites (polylinker: to allow for insertion of dna using different restriction endonucleases, by cutting and pasting, we can remove recognition sites from the body of plasmid to reduce sites we can add in dna. We kill others that do not contain the vector plasmid. Example of a plasmid vector this is the most adopted plasmid vector that contains two different. Colonies with vector and insert will be resistant to ampicillin and turn white (they won"t turn blue due to an insert in the plasmid). Isolate (or amplify) dna from donor organism and isolate vector (plasmid) dna: we can use pcr.
