Question

Which of the following is TRUE regarding transition state?

A

Option A: All of the above are true.

B

Option B: Transition state analogs bind the substrate not the enzyme

C

Option C: The transition state represents the ES complex.

D

Option D: The transition state can be stabilized by covalent catalysis.

E

Option E: The free energy between the transition state and the reactants must be negative.

Why can histidine NOT act as a base in the lysosome (an organelle where the pH is close to 4.5)?

A

Option A: Histidine would be protonated at pH 4.5.

B

Option B: Histidine has a positive charge at pH 4.5.

C

Option C: Histidine has a side chain pK close to 6.

D

Option D: None of the above is correct.

E

Option E: All of the above are correct

Acid-base catalysis may be accomplished by:

A

Option A: buffer effects

B

Option B: conformational change

C

Option C: active site specificity

D

Option D: charge delocalization

E

Option E: induced strain

Which type of catalysis may be carried out using redistribution of electron density to facilitate the transfer of a proton?
I. proximity
II. acid-base
III. covalent
IV. strain

A

Option A: I

B

Option B: II, IV

C

Option C: II, III

D

Option D: II

E

Option E: III

Which of the following processes would yield and increase in rate?

A

Option A: The rotational motions of the substrates and catalytic groups

B

Option B: None of the above

C

Option C: All of the above

D

Option D: The orientations of the substrates and catalytic groups

E

Option E: The proximity of the reacting groups

The catalytic mechanism of bovine pancreatic RNase A relies upon acid-base catalysis involving the amino acid __________.

A

Option A: lysine

B

Option B: histidine

C

Option C: glutamic acid

D

Option D: aspartic acid

E

Option E: imidazole