Which way does a neutral charged dye run? Positive charged dye? Negatively charged dye?
Which way does a neutral charged dye run? Positive charged dye? Negatively charged dye?
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10. When you make your agarose gel, you will need to make 50 ml of 1% (w/v) agarose in 1X TAE. How much agarose will you require? [you will make this later, not on Solution day] When adding your DNA samples to the agarose gel, you will need to add loading dye to the samples. Loading dye contains: ⢠glycerol, which helps the samples sink into the sample wells of the gel; and ⢠tracking dyes so that you have a way of estimating how far the samples have travelled through the gel during electrophoresis. The recipe for 6X loading dye is as follows: ⢠0.25% bromophenol blue (w/v) ⢠0.25% xylene cyanol (w/v) ⢠30% glycerol in water (v/v)
11. Provide a recipe for 10 ml of 10X loading dye, using the information above. (Important tip: Remember that you have already made 30ml of a 50% stock solution of glycerol. I recommend determining the concentration of glycerol required for 10X loading dye, then figuring out how much of your 50% glycerol stock will be needed to make 10ml of 10X loading dye.)
Solution to make | Exact pH of final solution (if appropriate) | Final container for solution | Completed (Y or N) |
100 ml of 0.9% (w/v) NaCl | |||
100 ml of 1.0M Tris, pH 7.6 | |||
40 ml of 50X TAE |
Molecular Biology Help!
Histone chaperones play a role in replication by:
Histone chaperones play a role in replication by:
preventing specific interactions between the positively charged histones and negatively charged DNA. | ||||||||||||||||||||
preventing nonspecific interactions between the positively charged histones and negatively charged DNA. | ||||||||||||||||||||
facilitating specific interactions between the positively charged histones and negatively charged DNA. | ||||||||||||||||||||
facilitating nonspecific interactions between the positively charged histones and negatively charged DNA. | ||||||||||||||||||||
none of the above. Histone chaperones play a role in replication by: Histone chaperones play a role in replication by:
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________________ may play a role in aging and cancer.
________________ may play a role in aging and cancer.
telomerase | |||||||||||||||
telomerase RNA | |||||||||||||||
DNA polymerase III | |||||||||||||||
restriction enzymes | |||||||||||||||
none of the above Telomeres consist of : Telomeres consist of :
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Which of the following is the eukaryotic DNA replication initiator?
Which of the following is the eukaryotic DNA replication initiator?
DnaA |
replication protein A |
replication protein C |
ORC |
MCM |
The yeast ARS element has which of the following functions and properties:
The yeast ARS element has which of the following functions and properties:
they are AT-rich and serve as replication termination sites |
they are GC-rich and serve as replication termination sites |
they have random sequence and serve as replication termination sites |
they are AT-rich and serve as replication initiation sites |
they are GC-rich and serve as replication initiation sites |
Replication factor C plays which of the following roles in eukaryotic DNA replication?
Replication factor C plays which of the following roles in eukaryotic DNA replication?
it is the sliding clamp | |||||||||||||||
it is the clamp loader | |||||||||||||||
it is the DNA ligase | |||||||||||||||
it is a single-stranded DNA binding protein | |||||||||||||||
it is a cofactor required for activating the DNA polymerase Which of the following is the replicative DNA polymerase for both the leading and lagging strands of SV40? Which of the following is the replicative DNA polymerase for both the leading and lagging strands of SV40?
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