Question

Microbiology

1) For gram positive cell walls, does the cell wall itself carry a negative charge and regulate movement of cations or is those characteristics of teichoic acid?

2) For basic dyes, it's a cation that stains a negatively charged microorganism?

3) Is the sole purpose for gram staining to differentiate between species?

4) Is carbolfuchsin a basic or acidic dye?

5) in special staining, do all tests (capsule, flagella, and endospore) show the halo effect?

6) in acid-fast staining, is there any interaction of charges?

7)the interaction between crystal violet and gram (-) bacteria. I assumed since gram (-) bacteria don't hold the dye, they do not interact. Can you explain how they do?