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1. what is considered a high concentration of carbon in the environment? 2. what is the most reduced form of oxygen? what is the most oxidized? 3.what is the most reduced form of nitrogen? what is the most oxidized? 4. what can be a sourse of phosphorous for bacteria? or sulfur? 5. if you are making 1 liter of media that requires 1.8 agar, how many grams of agar power do you add? 6. give an example of organic and inorganic nitrogen. 7. if you have dehydrated nutrint broth, what ingredient is required in order to make plates for streaking. 8. explain what its enrichment? what steps did you take in order to enrich for your specific bacteria. 9. if you have dehydrated skim milk agar, what ingredients is required in order to make petri plates for streaking.

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Nelly StrackeLv2
12 May 2019

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Related questions

Question 1

A microorganism's ability to use a harmful pesticide as a source of carbon and energy is an example of

Select one:

a. chemoautotrophy

b. nitrogen fixation

c. bioremediation

d. photoautotrophy

e. biosynthesis

Question 2

Autotrophs and heterotrophs differ in

Select one:

a. their ability to utilize CO2 as a sole carbon source.

b. their ability to incorporate CO2 into cells.

c. only a few enzymes.

d. their ability to synthesize precursor molecules.

e. their ability to utilize CO2 as a sole energy source.

Question 3

If the amino acid valine constituted approximately 4% of the total cell protein of a bacterium, how much valine should be added to a defined medium in order to grow 1 gram of this organism?

Select one:

a. 0.2 mg

b. 2 mg

c. 20 mg

d. 40 mg

e. 200 mg

Question 4

Transport of HPO4 along with a proton (H+) is an example of

Select one:

a. antiport

b. uniport

c. facilitated diffusion

d. symport

e. group translocation

Question 5

The energy required for transport via ABC transporters is derived from

Select one:

a. a proton gradient

b. an electrical charge separation across the membrane

c. ATP hydrolysis

d. a symport mechanism

e. a high energy phosphate bond of PEP

Question 6

The spread plate method would clearly be preferred over the pour plate method when isolating colonies of

Select one:

a. anaerobic bacteria

b. thermophilic bacteria

c. bacteria from temperate lake zones

d. purple non-sulfur bacteria

e. agar-degrading bacteria

Question 7

Enrichment culture procedures for many microorganisms require intial growth in liquid media, followed by growth on solid media. The solid media is used to

Select one:

a. provide proper growth conditions.

b. isolate single colonies of the microbes.

c. get rid of competing microorganisms.

d. isolate strict aerobes.

e. enrich for specific nutrients.

Question 8

The optical density of light scattered by bacteria is a very useful tool for estimating population size. What can alter the bacteria's light-scattering properties?

Select one:

a. Cell volume depending on its growth stage

b. The condition of being absent of microorganisms.

c. The amount of light projected from the room.

d. The standard curve

e. Standard deviation from growth curve.

Question 9

What is true about a bacterial colony?

Select one:

a. It arises from an individual cell and represents an isolate strain.

b. Colonies, and CFU (colony forming units) on streak plates represent a diverse microbial culture.

c. In order to obtain a colony, on must perform cloning steps.

d. Axenic cultures to not represent CFUs or colonies.

e. Colonies require differential media in order to separate them from the population.

Question 10

What is a biofilm?

Select one:

a. All cells in a population achieve a steady state

b. A specialized, surface-attached, collaborative community of bacteria

c. A complex media made of the chemical building blocks that a cell needs

d. A type of anaerobic growth chamber.

e. A type of spore

5. Some bacteria live in deep sea vents where no light penetrates, yet are still capable of producing their own reduced carbon (e.g. fixing carbon dioxide). These organisms are classified as

(A) photoheterotroph (B) photoautotroph

(C)chemoheterotroph (D)chemoautotroph

(E) organotroph

6. Nitrogen fixation is the conversion of

(A) ammonium ion to nitrogen gas. (B) nitrogen gas to ammonium ion.

(C)nitrate ion to nitrogen gas. (D)ammonium ion to nitrate ion.

(E) nitrate ion to ammonium ion.

7. Which of the following is true of complex media?

(A) Bacteria grow faster on them than on synthetic media.

(B) All the chemical components and their concentrations are known.

(C)Complex media are the most difficult of all to prepare.

(D)Complex media can help determine the metabolic capacity of an organism.

(E) They are also called minimal media.

8. Which of the following types of growth media contains blood?

(A) minimal media (B) complex media

(C)defined media (D)enriched media

(E) selective media

9. The bile salts and crystal violet in MacConkey lactose plates prevent the growth of Gram-positives. These additives make the MacConkey plate a

(A) complex medium. (B) defined medium.

(C)minimal medium. (D)selective medium.

(E) differential medium.

10. You discover a new bacterial efflux pump that moves an uncharged toxin out of the cell using the proton motive force as the energy source. This new transporter can be classified as an

(A) electrogenic antiporter (B) electrogenic symporter

(C)electroneutral antiporter (D)electroneutral symporter

(E) none of the above

11. Which of the following involves nitrate as a terminal electron acceptor?

(A) photosynthesis (B) aerobic respiration

(C)fermentation (D)anaerobic respiration

(E) glycolysis

12. Which of the following is least likely to use glucose (an organic molecule) as a source of electrons?

(A) lithotrophy (B) organotrophy

(C)aerobic respiration (D)anaerobic respiration

(E) heterotrophy

17. Which of the following nutrient uptake systems does not require cellular energy?

(A) facilitated diffusion (B) coupled transport

(C)ABC transporters (D)group translocation

(E) All of the above require energy expenditure by the cell

18. What is the energy source for ABC transporters?

(A) proton motive force (B) NADH

(C)phosphoenolpyruvate (PEP) (D)ATP

(E) No energy is required for ABC transport.

19. Which of the following is true of all biofilms?

(A) They only contain a single species of bacteria.

(B) Biofilms can form on a range of biotic or abiotic surfaces.

(C)Cells in a biofilm are dormant until the bacteria leave the biofilm.

(D)Biofilms are composed of a single monolayer of EPS.

(E) Biofilms are not found in or on the human body.

20. The heterocyst cells of cyanobacteria are specialized for

(A) photosynthesis (B) gliding motility

(C)nitrogen fixation (D)carbon fixation

(E) antibiotic production

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