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  Brightfield Microscope Problem 2 a) Using a blue light filter with a wavelength of 400 nm, calculate the resolution ‘R’ for the oil immersion lens in μm.  b) Explain in words what this value means in terms of resolution

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Related questions

. On a given microscope, the numerical apertures of the condenser and low power objective lenses are 1.25 and 0.25, respectively. You are supplied with a filter that selects a wavelength of 520nm.

a.) What is the limit of resolution on this microscope? Please be specific to how you came up with this answer, what formula did you use? b.) Will you be able to distinguish 2 points that are 300nm apart as being separate, or will they blur into one?

on the same microscope as in Question#5, the high dry objective lens has a numerical aperture of 0.85, what is the limit of resolution on this microscope, and will you be able to distinguish 2 points that are 300 nm apart as being separate, or will they blur into one? Explain answer.

5. On a given microscope, the numerical apertures of thecondenser and low power objective lenses are 1.25 and 0.25,respectively. You are supplied with a filter that selects awavelength of 520nm.

a.) What is the limit of resolution on this microscope? Pleasebe specific to how you came up with this answer, what formula didyou use? b.) Will you be able to distinguish 2 points that are300nm apart as being separate, or will they blur into one?

6. on the same microscope as in Question#5, the high dryobjective lens has a numerical aperture of 0.85, what is the limitof resolution on this microscope, and will you be able todistinguish 2 points that are 300 nm apart as being separate, orwill they blur into one? Explain answer.

This is a confocal microscopy class. So please answer clear and detail according to the subject. Thank you

1.Explain how light diffraction causes the lateral point spread function of light as shown by Airy. Illustrate with a diagram of the PSF in the lateral direction.

2.What is the relative size of the point spread function in the vertical compared to lateral direction and how does this affect the shape of the voxels we record in a optimal resolution z-stack.

3.Is the point spread function wider or narrower with large aperture lenses? Explain why.

4.Does a larger aperture lens have higher resolution (more detail) or lower resolution (poor detail)? Or is aperture size unrelated to resolution?

5.Definedynamicrange.Explaintheimportanceofadjustingthedynamicrangeof the light intensity sensors (PMTs) and how the confocal encodes intensity into numbers.

6.Explain the advantage and disadvantage of frame-averaging.

6b.Explain how to optimize the resolution of your scanned image to avoid oversampling and minimize file size.

7.Make a list of the most functionally-important parts inside of the scan head box and briefly explain what each part does. Hint1: there are 5 kinds of things, and the AOTFs & lasers are not in the scan head box. Hint2: Without these 5 things, it would not be a laser scanning confocal microscope.

8.Explain why immersion oil is used for some lenses. Explain how it works.

9.Explain the relationship between the point-spread function and super-resolution.

10. What is chromatic aberration?

11. How does one detect cross-talk and bleed-through? How can you avoid it, and how can you correct for it if you could not avoid it?

12. Describe the three main ways to manage autofluorescence.

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