. On a given microscope, the numerical apertures of the condenser and low power objective lenses are 1.25 and 0.25, respectively. You are supplied with a filter that selects a wavelength of 520nm.
a.) What is the limit of resolution on this microscope? Please be specific to how you came up with this answer, what formula did you use? b.) Will you be able to distinguish 2 points that are 300nm apart as being separate, or will they blur into one?
on the same microscope as in Question#5, the high dry objective lens has a numerical aperture of 0.85, what is the limit of resolution on this microscope, and will you be able to distinguish 2 points that are 300 nm apart as being separate, or will they blur into one? Explain answer.
. On a given microscope, the numerical apertures of the condenser and low power objective lenses are 1.25 and 0.25, respectively. You are supplied with a filter that selects a wavelength of 520nm.
a.) What is the limit of resolution on this microscope? Please be specific to how you came up with this answer, what formula did you use? b.) Will you be able to distinguish 2 points that are 300nm apart as being separate, or will they blur into one?
on the same microscope as in Question#5, the high dry objective lens has a numerical aperture of 0.85, what is the limit of resolution on this microscope, and will you be able to distinguish 2 points that are 300 nm apart as being separate, or will they blur into one? Explain answer.
Related textbook solutions
Related questions
QUESTION 1
The ocular of a compound microscope has a magnification of 10x and the objective has a magnification of 10x. The total magnification of the microscope is ___---
| a. | 10x | |
| b. | 20x | |
| c. | 100x | |
| d. | 1000x |
QUESTION 2
The part of the microscope that focuses the light on an object is the ___________.
| a. | Iris Diaphragm | |
| b. | Coarse Focus | |
| c. | Condenser | |
| d. | Body Tube |
QUESTION 3
The polarizing filter in a PLM that is located above the objective is called the _________________.
| a. | Polarizer | |
| b. | Analyzer | |
| c. | Abbe condenser | |
| d. | Iris |
QUESTION 4
A substance that reacts the same to light polarized in any direction is _________________.
| a. | an isotope | |
| b. | anisotropic | |
| c. | isotropic | |
| d. | divergent |
QUESTION 5
The part of the comparison microscope that allows the examiner to view two objects simultaneously is called the _________________.
| a. | comparator | |
| b. | comparison bridge | |
| c. | spectroscope | |
| d. | stage |
1 points
QUESTION 6
A stereo microscope can best be described as _____________________.
| a. | Two compound microscopes aligned so that they each see a slightly different part of an object. | |
| b. | Two compound microscopes aligned with a comparison bridge. | |
| c. | A compound microscope with two separate stages and one ocular. | |
| d. | A compound microscope with two eyepieces and a camera mount. |
QUESTION 7
In microscopy, resolution is a measure of ________________.
| a. | The ability of the lenses to separate two tiny details that are close together. | |
| b. | The total magnification power of the microscope. | |
| c. | The empty magnification power of the microscope. | |
| d. | The ability of an electron microscope to determine the presence of a large number of elements. |
QUESTION 8
Two polarizers (polarizing filters) aligned 90° from each other will block out all light.
True
False
QUESTION 9
A simple convex lens creates a magnified real image of an object.
True
False
QUESTION 10
A trinocular microscope has three objective lenses mounted on a turret.
True
False
QUESTION 11
Liquids and gases are usually isotropic.
True
False
QUESTION 12
Match each of the following terms with Its Definition
Compound microscope a. Has a polarizer and an analyzer
Electron microscope b. Permits viewing two objects at once
Stereomicroscope c. Consists of a single convex lens
Comparison microscope d. Long working distance, three-dimensional
Simple hand magnifier e. Basic 2 convex lens microscope
Polarizing microscope f. Can magnify images more than 100,000 times
