What feature of Agrobacterium allows it to insert it's DNA into a plant?

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Casey DurganLv2

16 Jul 2019

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Q1.

You are a genetic engineering hired by the biotechnology company, Genes 'R' Us, to use genetic engineering to develop a new rose that glows. Place the following steps in the order you would need to perform them to successfully genetically engineer the rose with this trait.

-Extract all of the DNA from a firefly cell

-Use traditional plant breeding techniques to improve the performance of the rose, which has the new glowing gene (transgene)

-Clone the single gene that encodes the glowing protein

-Insert the glowing gene into single rose plant cell using either the gene gun or agrobacterium

-Modify the glowing gene so it acts In the way you want it once its inside the rose plants

-Identify the firefly as an organism that would have a gene encoding a protein for a âglowing traitâ

Q2.

Genetic engineering is different from traditional plant breeding in that (select all that apply)

___genetic engineering 'physically' removes DNA (genes) from one organism and inserts them into another

___plant breeding and classical biotechnology relies on natural sexual reproduction to create variation while genetic engineering introduces variation one or a few genes at a time into a single cell.

___genetic variation used in classical biotechnology (plant breeding) comes from mutations in genes already found in that crop species. In genetic engineering, new genes are added to the genetic makeup of a plant

___genetic engineers can move DNA from any organism into any organism, even if they are not in the same species.

Q3.

Which method of plant transformation works and depends upon an organism that is a "naturally occurring" genetic engineer?

A)Gene Gun

B)Agrobacterium

C)Microneedle insertion into a plant egg cell

D)Electric Shock of Plant Cells

rubydeer239

What feature of DNA methylation allows it to be maintained through DNA replication?

The CpG configuration

Restriction endonuclease sites

Histone modification

The Xist gene

raspberrybat503

A new operon is discovered in Agrobacterium tumefaciensthat allows the bacteria to break down contaminants in the soil.The operon is transcriptionally active only when both contaminantsand root exudates are present in the soil. All you know about theoperon is that it is in a genomic region of 24kb that is betweentwo restriction sites (NotI and SfiI). The process of contaminantbreak down happen in stages: 1) the soil