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6 Jan 2018
Is it true or false that one can produce large quantities of human growth hormone by PCR cloning of the full-length GH1 gene from human chromosome 17 between positions base pair 61,994,563 to base pair 61,996,198, then purifying the PCR product on agarose gel and ligating the purified 1636 base-pair long amplicon into a suitable expression vector, which is then placed into E. coli? You may assume that glycosylation differences between prokaryotes and eukaryotes are not important when answering this question.
Is it true or false that one can produce large quantities of human growth hormone by PCR cloning of the full-length GH1 gene from human chromosome 17 between positions base pair 61,994,563 to base pair 61,996,198, then purifying the PCR product on agarose gel and ligating the purified 1636 base-pair long amplicon into a suitable expression vector, which is then placed into E. coli? You may assume that glycosylation differences between prokaryotes and eukaryotes are not important when answering this question.
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