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THREE MICROBIOLOGY QUESTIONS:

1. What general characteristics differentiate non-glucose fermenters from the Enterobacteriaceae?

2. List the common patient risk factors for diseases caused by non-fermentative gram negative rods.

3. Differentiate between the Enterobacteriaceae and non glucose fermenters with the TSI reaction.

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Tod Thiel
Tod ThielLv2
28 Sep 2019

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Related questions

9. An oxidase-positive, wrinkled, non-lactose fermenting colony is suspicious for

a. the organism that causes glanders.

b. an individual with cystic fibrosis.

c. a potential bioterrorism agent.

d. a common antibiotic-resistant nosocomial organism.

10. The isolation of an extremely mucoid, oxidase-positive, non-lactose fermenter from the lower respiratory tract usually correlates with the clinical diagnosis of

a. melioidosis.

b. nothing—it is an environmental contaminant.

c. glanders.

d. cystic fibrosis.

11. Which of the following gram-negative rods is associated with individuals who have cystic fibrosis?

a. An oxidase-positive, pyocyanin-producing, non-lactose fermenter.

b. An oxidase-negative, nonmotile, non-lactose fermenter that is K/NC on TSI.

c. An oxidase-positive, lysine-positive, non-lactose fermenter.

d. a and c.

e. All of the above.

12. Two blood cultures of a premature infant grow a yellow-pigmented colony that is a non-lactose fermenter. The colony provides the following reactions:

Oxidase: purple Indole: pink Motility: negative

The organism is probably

a. Burkholderia cepacia complex.

b. Stenotrophomonas maltophilia.

c. Elizabethkingia meningosepticum.

d. none of the above.

13. An anemic patient received a unit of blood. She develops fever and chills after infusion of the unit. Blood cultures of the patient and a sample of the blood unit grow the same organism. The non-lactose fermenter isolated displays the following reactions:

Oxidase: purple Growth at 42°C: negative Colony under UV light: yellow Arginine: purple Motility: positive Gelatin hydrolysis: liquified Acetamide: no color change Nitrate after addition of zinc: red

The organism is

a. Pseudomonas. putida

b. P. fluorescens.

c. P. aeruginosa.

d. P. stutzeri.

14. Which of the following oxidase-negative, non-motile, non-fermenting, gram-negative rods can mimic a late lactose fermenter due to the oxidation of lactose rather than fermentation?

a. Acinetobacter baumannii

b. Burkholderia cepacia complex

c. Achromobacter xylosoxidans

d. Stenotrophomonas maltophilia

15. A physician calls the microbiology department for the results of a lower respiratory culture of a 15-year-old female with cystic fibrosis. The culture grows an oxidase-negative, motile, lysine-positive, non-lactose fermenter that presents as K/NC on TSI and an oxidase-positive, lysine-positive, non-lactose fermenter. Based on these results, you tell the physician she can potentially treat both organisms with

a. imipenem.

b. an aminoglycoside.

c. polymyxin B.

d. trimethoprim-sulfamethoxazole.

16. An oxidase-negative, non-motile, non-lactose fermenter demonstrates K/NC on TSI and is considered a significant nosocomial pathogen due to its multiresistance. Which of the following antibiotics remains a viable treatment option for this organism?

a. Imipenem

b. Piperacillin/tazobactam

c. Aztreonam

d. Ciprofloxacin

17. A tracheal aspirate, obtained from a comatose patient on a ventilator, grew an oxidase-positive non-lactose fermenter that produced a distinctive odor of sliced apples. It proved to be:

Motile: positive Nitrate: red after addition of reagents A, B, and zinc OF medium: open tubes: glucose: blue, maltose: blue, lactose: blue, sucrose: blue

closed tubes: glucose: green, maltose: green, lactose: green, sucrose: green

This organism is

a. Acinetobacter baumannii.

b. Alcaligenes faecalis.

c. Moraxella nonliquefaciens.

d. Elizabethkingia meningosepticum.

.1 List the signs and symptoms of sepsis, and explain the most common types of infections that develop in to septic shock. Make sure to include information to differentiate between gram-positive sepsis and gram-negative sepsis. Lastly, describe what the two conditions are that define the systemic inflammatory response syndrome of sepsis.

2)

. List a viral, bacterial, and fungal microbe that can cause a respiratory infection. With these three microbes, include the following for each (16 points):

a. mode of transmission

b. disease symptoms

c. preferred treatment

d. a laboratory test that could be done to determine a patient has the microbe

Answer the two questions at the end of the lab please :3

MATERIALS
• Sterile cotton swab • Oxidase reagent • Cultures (agar slant): • Positive Control: Pseudomonas aeruginosa • Negative Control: Escherichia coli
PROCEDURES
1. Obtain a small sample of microbe on the tip of a sterile cotton swab
2. Place two drops of oxidase reagent over the sample
3. Observe the color change
A positive reaction may appear pink at first, then maroon and finally black/dark blue. Take care to avoid contact with the oxidase reagent. NOTE: An alternate procedure is performed by placing some oxidase reagent directly on the colony on the agar or on organisms applied to a filter disk.


Questions
1. What is the method for reduction of oxygen in Enterobacteriaceae?
2. How is ATP generated as a result of electron transport systems?

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