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Answer the two questions at the end of the lab please :3

MATERIALS
• Sterile cotton swab • Oxidase reagent • Cultures (agar slant): • Positive Control: Pseudomonas aeruginosa • Negative Control: Escherichia coli
PROCEDURES
1. Obtain a small sample of microbe on the tip of a sterile cotton swab
2. Place two drops of oxidase reagent over the sample
3. Observe the color change
A positive reaction may appear pink at first, then maroon and finally black/dark blue. Take care to avoid contact with the oxidase reagent. NOTE: An alternate procedure is performed by placing some oxidase reagent directly on the colony on the agar or on organisms applied to a filter disk.


Questions
1. What is the method for reduction of oxygen in Enterobacteriaceae?
2. How is ATP generated as a result of electron transport systems?

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Hubert Koch
Hubert KochLv2
28 Sep 2019

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