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A synthetic lethal interaction is one in which two yeast stains with non-lethal phenotypes are crossed and the resulting double mutant is lethal.

Of these yeast strains, what are all the possible combinations that could be crossed to look for synthetic lethal interactions?

Strain A: a null mutation in a nonessential gene
Strain B: a null mutation in an essential gene
Strain C: a conditional mutation in an essential gene

Select all that apply.

Select all that apply.

A. strain A x strain A
B. strain B x strain B
C. strain C x strain C
D. strain A x strain B
E. strain A x strain C
F. strain B x strain C

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Nestor Rutherford
Nestor RutherfordLv2
28 Sep 2019

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Related questions

Flies with a dominant mutation in a particular gene have an additional set of wings. Through mapping techniques, you identify a candidate gene, gene X, on an autosomal chromosome that you think results in extra wings when mutated. When you sequence gene X in the mutant fly with extra wings, you notice a missense mutation in the middle of the coding region of gene X. What is the best way to verify that the mutation in gene X is responsible for the dominant phenotype?

a. Starting with a wild-type fly, knockout gene X to generate a null allele and see if flies that are heterozygous or homozygous for the null allele have extra wings.

b. Starting with the mutant fly that is heterozygous for the dominant allele, insert a wild type copy of gene X (complete with its own promoter) elsewhere in the genome of the fly and see if it rescues the mutant and results in normal/wild-type wings.

c. Starting with a mutant fly that is heterozygous for the dominant allele, replace the dominant mutant allele with a wild type version of the gene via homologous recombination and see whether flies with the replacement have normal/wild type wings.

d. Starting with a homozygous wild-type fly, replace one copy of wild-type gene X with a version carrying the dominant mutation via homologous recombination, and see whether flies with the replacement have extra wings.

e. either C or D

You have a strain of flies that are homozygous for a deletion of 2 neighboring genes, A and B (genotype aabb). Wild type flies have transparent wings but these aabb flies have rainbow-colored wings. Which of the following experiments would provide you with more information about which of these genes (if either) is responsible for the rainbow-colored wings?

a. Starting with a wild-type fly, create a transgenic fly that is homozygous for deletion in both genes A and B and see if you get the rainbow-colored wings.

b. Delete gene A alone and gene B alone in 2 independent wild-type flies and see whether flies homozygous for either deletion have rainbow-colored wings.

c. Starting with a mutant fly with rainbow-colored wings, introduce both wildtype genes A and B into a new place in the genome of the fly and see if normal wing color is restored.

d. non of the above

Canaries: Green (wild-type) Pied phenotype Yellow type

Canaries are popular pets and easy to breed in captivity. Additionally, they display great phenotypic diversity, which allows you to do some interesting genetic investigations with them.

When you take a canary from a true-breeding green strain and cross it with a canary from a true-breeding yellow strain, all offspring display the pied phenotype. Based on this you suspect that this color variation is inherited by a gene with a co-dominant inheritance pattern (showing both phenotypic traits in hybrid phenotypes).

You decide to test this hypothesis. To do so, you take pairs of your hybrid F1 individuals (of pied phenotype), and cross them to produce an F2 generation.

After many dozens of crosses and hundreds of offspring, you arrive at the peculiar ratio of:

3/16 green : 6/16 pied : 7/16 yellow

Which of the following hypotheses are consistent with these data? (Note that not all of these hypotheses are mutually exclusive. Assume that the two genes are independently assorting)

Hint: This problem is very hard to work from the given ratios to what is happening genetically. It is easier to "work backwards" and start with each choice below, and see if you can obtain all or important aspects of the results of your cross when making the assumption that the answer choice is correct.

Select all that apply:

Select one or more:

a. At least one of the genes involved is sex linked.

b. One gene controls production of darker pigment (melanin which appears green when mixed with yellow). A second gene acts as a patterning gene with one allele causing expression and the other non-expression of melanin and when in the heterozygous condition each allele controls melanin expression on different parts of the body so long as the bird's genotype of the first gene allows for the production of melanin.

c. this is a single-gene trait with co-dominance

d. one of the two genes involved displays complete dominance, while the other displays co-dominance.

e. the genes involved are epistatically interacting

f. more than one gene is involved

2.You are a first year graduate student in a genetics lab. When you come into the lab, the principal investigator hands you a mutant strain and says, “Come back tomorrow, and tell me how you would clone this gene.” Describe the strategy and steps that would be used to clone the gene responsible for each of the following mutant phenotypes, and to convince someone that you have cloned the correct gene. Assume that all of the mutant phenotypes are recessive and can be grown as homozygotes. You may also assume that you have access to all of the information and genetic strains and reagents that are widely used in that organism. However, at the time the analysis begins, you have only the mutant (and access to the genome databases), and no other information. a. A yeast mutant that results in hyphal growth – that is, long filaments rather than discrete round colonies. b. A mutant in flies that results in crossing over in males but has no effect in females. (Recall that males in Drosophila do not typically cross over during meiosis.) c. A mutant in worms that forms blisters on the cuticle. The cuticle is made of collagen. d. An Arabidopsis mutant that flowers much earlier than the wild-type under normal lab or green house conditions. e. A mouse with a variegated coat color pattern (such as spots or blotches) rather than a solid black or brown color.

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