2.You are a first year graduate student in a genetics lab. When you come into the lab, the principal investigator hands you a mutant strain and says, âCome back tomorrow, and tell me how you would clone this gene.â Describe the strategy and steps that would be used to clone the gene responsible for each of the following mutant phenotypes, and to convince someone that you have cloned the correct gene. Assume that all of the mutant phenotypes are recessive and can be grown as homozygotes. You may also assume that you have access to all of the information and genetic strains and reagents that are widely used in that organism. However, at the time the analysis begins, you have only the mutant (and access to the genome databases), and no other information. a. A yeast mutant that results in hyphal growth â that is, long filaments rather than discrete round colonies. b. A mutant in flies that results in crossing over in males but has no effect in females. (Recall that males in Drosophila do not typically cross over during meiosis.) c. A mutant in worms that forms blisters on the cuticle. The cuticle is made of collagen. d. An Arabidopsis mutant that flowers much earlier than the wild-type under normal lab or green house conditions. e. A mouse with a variegated coat color pattern (such as spots or blotches) rather than a solid black or brown color.
2.You are a first year graduate student in a genetics lab. When you come into the lab, the principal investigator hands you a mutant strain and says, âCome back tomorrow, and tell me how you would clone this gene.â Describe the strategy and steps that would be used to clone the gene responsible for each of the following mutant phenotypes, and to convince someone that you have cloned the correct gene. Assume that all of the mutant phenotypes are recessive and can be grown as homozygotes. You may also assume that you have access to all of the information and genetic strains and reagents that are widely used in that organism. However, at the time the analysis begins, you have only the mutant (and access to the genome databases), and no other information. a. A yeast mutant that results in hyphal growth â that is, long filaments rather than discrete round colonies. b. A mutant in flies that results in crossing over in males but has no effect in females. (Recall that males in Drosophila do not typically cross over during meiosis.) c. A mutant in worms that forms blisters on the cuticle. The cuticle is made of collagen. d. An Arabidopsis mutant that flowers much earlier than the wild-type under normal lab or green house conditions. e. A mouse with a variegated coat color pattern (such as spots or blotches) rather than a solid black or brown color.
