1)Urea broth was prepared and sterilized by autoclaving. Bacteria was inoculated and after an overnight incubation, the color was unchanged. What conclusion can you make from this result?
2)When un-inoculated Simmons Citrate media was incubated overnight, it turned blue. Explain this color change.
3)For a unknown, your results are the following: Gram negative rod; turbidity and yellow color in Phenol Red Lactose; Turned blue in Simmonâs Citrate agar; Urea broth turned pink; Methyl Red negative; Vogues-Proskauer positive; Kovacâs reagent negative; non-motile on motility tube. What is this unknown?
4) According to Table 39.3 in the Lab Manual, K. pneumonia bacteria is citrate Positive. Explain how and why a strain of K. pneumonia would result in a citrate negative result.
1)Urea broth was prepared and sterilized by autoclaving. Bacteria was inoculated and after an overnight incubation, the color was unchanged. What conclusion can you make from this result?
2)When un-inoculated Simmons Citrate media was incubated overnight, it turned blue. Explain this color change.
3)For a unknown, your results are the following: Gram negative rod; turbidity and yellow color in Phenol Red Lactose; Turned blue in Simmonâs Citrate agar; Urea broth turned pink; Methyl Red negative; Vogues-Proskauer positive; Kovacâs reagent negative; non-motile on motility tube. What is this unknown?
4) According to Table 39.3 in the Lab Manual, K. pneumonia bacteria is citrate Positive. Explain how and why a strain of K. pneumonia would result in a citrate negative result.