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In the rare case where PCR-based DNA fingerprinting fails to demonstrate a single guilty suspect, what other techniques can be used to analyze DNA from a crime scene?

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Irving Heathcote
Irving HeathcoteLv2
28 Sep 2019

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Related questions

PCR is used regularly in criminal cases to compare the identity of a perpetrator to suspects. One way that this is done is based on the size of DNA amplified from a genome. Our genomes are full of “junk DNA” that does not encode genes. This DNA can vary greatly from person to person in both length and sequence.
Jane is a technician in a DNA evidence lab. She needs to compare the “junk DNA” from the site of a crime and a suspect. This is obviously an important job and she needs to do it correctly. Jane needs help setting up her experiment. She is already setting up a PCR with the DNA from the crime and DNA from the suspect, but she feels like she is missing something. Given the exceptional sensitivity of PCR and the fact that we all have DNA, including Jane herself, what are the reaction conditions of an additional PCR tube that Jane needs to set up? In other words, what should she add to another reaction to make sure she is only looking at DNA from either the crime scene or the suspect?

1. Why is MgCl2 included in the PCR buffer? MgCl2 is used in the PCR buffer because it help 2. If you started a PCR protocol with 10 double stranded molecular of DNA, how many double stranded molecules of product would you have after 5 cycles of PCR? 3. Where is the initiation and stop codon of the GFPuv gene in the forward and reverse primers, respectively? 4. What kinds of materials obtained from a crime scene might contain DNA, and where might you find them at a crime scene? 5. Why do you need to perform PCR on DNA obtained froma crime scene? 6. What is genotype? 7. What is the different between an allele and a locus? 8. What is an allele ladder? What is its function in DNA profiling? 9. Why do forensic labs analyze non-coding DNA and not genes?\ 10. How unit is defined for the restriction enzymes EcoRI and HindIII from New England BioLabs? 11. What is the star activity of restriction enzymes?

BTH 2732 Recombinant DNA Technology

A policeman had to investigate a homicide case involving a woman. The team managed to collect hair and semen samples from her body at the crime scene. How can PCR be used in the identification of the murderer?

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