1. Why is MgCl2 included in the PCR buffer? MgCl2 is used in the PCR buffer because it help 2. If you started a PCR protocol with 10 double stranded molecular of DNA, how many double stranded molecules of product would you have after 5 cycles of PCR? 3. Where is the initiation and stop codon of the GFPuv gene in the forward and reverse primers, respectively? 4. What kinds of materials obtained from a crime scene might contain DNA, and where might you find them at a crime scene? 5. Why do you need to perform PCR on DNA obtained froma crime scene? 6. What is genotype? 7. What is the different between an allele and a locus? 8. What is an allele ladder? What is its function in DNA profiling? 9. Why do forensic labs analyze non-coding DNA and not genes?\ 10. How unit is defined for the restriction enzymes EcoRI and HindIII from New England BioLabs? 11. What is the star activity of restriction enzymes?
1. Why is MgCl2 included in the PCR buffer? MgCl2 is used in the PCR buffer because it help 2. If you started a PCR protocol with 10 double stranded molecular of DNA, how many double stranded molecules of product would you have after 5 cycles of PCR? 3. Where is the initiation and stop codon of the GFPuv gene in the forward and reverse primers, respectively? 4. What kinds of materials obtained from a crime scene might contain DNA, and where might you find them at a crime scene? 5. Why do you need to perform PCR on DNA obtained froma crime scene? 6. What is genotype? 7. What is the different between an allele and a locus? 8. What is an allele ladder? What is its function in DNA profiling? 9. Why do forensic labs analyze non-coding DNA and not genes?\ 10. How unit is defined for the restriction enzymes EcoRI and HindIII from New England BioLabs? 11. What is the star activity of restriction enzymes?
