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Design a purification scheme to separate all of the peptides blow, using at least two of the methods we discussed in class sequentially. Assume you start with a homogenous solution containing all of the peptides in a buffer at pH 7.0. Explain in complete sentences WHAT you plan to do and HOW it will work to separate the peptides. Include which methods you will use and in what order, as well as the order in which the peptides will elute. You may want to include a diagram in addition to your description. (8 points)

1. DVE
2. LDQDAEEDNG

3. RLK

4. EVIEED
5. GFQVAIMGTP

6. KRKNHQ
7. VKWKIHAKSR

8. FSL
9. FLMFLA

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Sixta Kovacek
Sixta KovacekLv2
28 Sep 2019

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Related questions

the commonly used ion exchange DEAE (Diethyl-amino-ethyl) at neutral pH. You want to separate the following mixture of 3 polypeptides on a DEAE column: Peptide 1: MSRNVIKDCKTERYF Peptide 2: MEQNVNQDCKTERYD Peptide 3: MKDHSELDCEHEDHF What will be the order in which these peptides elute from the column as you progressively increase the concentration of KCl in pH 7.0 buffer applied to the column. To keep it simple, we'll just refer to low salt; medium salt; high salt eluted fractions.

A. Peptide 1 low salt; Peptide 2 medium salt; Peptide 3 high salt.

B. Peptide 1 low salt; Peptide 3 medium salt; Peptide 2 high salt.

C. Peptide 2 low salt; Peptide 1 medium salt; Peptide 3 high salt.

D. Peptide 2 low salt; Peptide 3 medium salt; Peptide 1 high salt.

E. Peptide 3 low salt; Peptide 1 medium salt; Peptide 2 high salt.

F. Peptide 3 low salt; Peptide 2 medium salt; Peptide 1 high salt.

For the first part, you will write three different effective learning objectives focusing on insects that will support the standard from your discussion. For the second part, you will search for an existing insect-based lesson plan on the internet. Identify the standards addressed in that lesson and re-write your own learning objectives for the lesson plan.

Things to remember about learning objectives:

Learning objectives are brief descriptions of specific things a learner completing the lesson will know or be able to do. They should be succinctly expressed using clear action verbs.

The text of effective instructional objectives includes three parts. These parts are best described in the work of training and human performance expert Robert Mager:

description of a performance - Question to ask when writing this part: What do I want students to be able to do? The performance must be observable so be specific (E.g., Don't simply state students will understand something. You can't observe that. Students will define in writing, sort insects into groups by order, etc.).

conditions - important conditions under which the performance is expected to occur Examples: Given a list of... (sort into stacks); when provided with standard tools... (construct a table); without using references... (know the state capitols) Questions to ask when writing this part: What are the important conditions or constraints under which I want them to perform? What the learner will be provided? What will the learner be denied? Are there special conditions which occur on the job or when performing?

criterion or standard - the quality or level of performance that will be considered acceptable. Examples: include measures of speed (in less than 30 minutes...), direction (according to manufacturers specifications...), accuracy (without error...) , quality (all cuts must be smooth to the touch). Question to ask when writing this part: How well must learners perform for me to be satisfied they've accomplished the objective?


Part 1 must include:

1.The grade level, the Big Idea/Supportive Idea, and Next Generation Sunshine State Standard (5 points).
2. Three effective learning objectives (see advice above) that focus on insects and support that standard (5 points a piece).

Part 2 includes:

1.A working link for the lesson plan (2 points).
2. The grade level, the Big Idea/Supportive Idea, and Next Generation Sunshine State Standard (3 points).
3. Three effective learning objectives that focus on insects and support that standard (5 points a piece).

Water and Minerals

COLLAPSE

This discussion requires knowledge of the material from the chapter and research from online databases or valid research sources. This is truly an excellent learning experience for all of us.

Cancer and Chronic Disease (parkinson's, alzeimers, MS, CVD, auto-immune etc) are words that scare most of us. Certainly when it comes to prevention research shows that smoking, excessive alcohol use, exposure to toxins, obesity, poor diet, lowered immunity, inflammation are all implicators. We can definitely reduce our risk with good lifestyle choices (not smoking, normal BMI, etc.) and that includes dietary choices. There is quite a bit of research and debate about which minerals are beneficial for cancer and/or disease prevention (if at all) and research changes often. You can also focus on water, this will probably lead you down a track to discussing the effects of certain pollutants in the water supply. In any case we are going to find that research and share the knowledge!

This week, for your original posting, find a scholarly based article, professional journal article, original research from grey literature (www.greylit.org) or well respected organization/association research on diet and cancer or chronic disease prevention. This could include the presence of minerals, as supplements or from food, or something related to the water supply and cancer/chronic disease prevention, special diet for cancer/chronic diseases prevention etc. It can be specific to a particular type of disease or cancer or more general. If you select a chronic disease, select one specific one. We are on the minerals chapter, so I would like to see research focused on a mineral or that includes a mineral or multiple minerals. (for example though, don't research a high protein diet as that is a macronutrient).

What to consider:

1. Two wasy to navigate sources for you: get on Google scholar: https://scholar.google.com or for non-published research you can query: www.greylit.org OR have the MC librarian direct you to the various databases you have access too (you can call MC library and they will help you if you are fully online and don't want to go in). Absolutley NO wikipedia, livestrong or webmd, Mayo type sites. You can start here for topic ideas but look further! We are looking for scholarly research. Professional organizations are also a good place as well (for instance, I am interested in digestive conditions, and utilize the American Gastroenterological Association site which links to their journal).

2. find something you are interested in and use that as the basis of your search. This could be information from chapter 8. Also remember, it does not just have to be cancer, you can select any chronic condition you are interested in and see if there is research related to vitamins, minerals or phytochemicals.

3. **In many cases on Google Scholar you can see several version of an article and often you can access a free PDF version of the full article. If NOT, call the MC library and ask for our college log on and see if we have rights to the article. They may have given this info to you but I have found most students have to ask. OR the MC library will actually retrieve the full text article for you and email you the PDF, utilize the librarians!! They love it. **do NOT pay to purchase a full text article online! Utilize the MC library!!

3. If you find an article of interest in a regular magazine or paper (print or online) like Health magazine online, New York times, Wall street journal etc. and you like it, then at the END of the article or referenced IN the article will be information on the original research where the writer got the information. You can discuss the general article in your research but also find the original scholarly article online! **include both in your original posting as attachments or links.

For your original posting:

1. in the Subject box put the topic you researched i.e. lead contaminated water and the effects of cognitive development

2. Then post your article (and include either a link or preferabley the pdf or word doc so we can see the entire article). and also provide a detailed summary of the information you read about and if you agree or disagree with the information. Also discuss why you selected this particular article. **document the source appropriately, I am not concerned if you use APA or MLA.

3.**IMPORTANT, for full points YOU are summarizing what the article/research study said in your own words and discussing merits, issues, if you agree, disagree etc. I am having you attach the article/research study you referenced instead of just a works cited info so others in the class can access the information as well. **you are not cutting and pasting the abstract!!

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