the commonly used ion exchange DEAE (Diethyl-amino-ethyl) at neutral pH. You want to separate the following mixture of 3 polypeptides on a DEAE column: Peptide 1: MSRNVIKDCKTERYF Peptide 2: MEQNVNQDCKTERYD Peptide 3: MKDHSELDCEHEDHF What will be the order in which these peptides elute from the column as you progressively increase the concentration of KCl in pH 7.0 buffer applied to the column. To keep it simple, we'll just refer to low salt; medium salt; high salt eluted fractions.
A. Peptide 1 low salt; Peptide 2 medium salt; Peptide 3 high salt.
B. Peptide 1 low salt; Peptide 3 medium salt; Peptide 2 high salt.
C. Peptide 2 low salt; Peptide 1 medium salt; Peptide 3 high salt.
D. Peptide 2 low salt; Peptide 3 medium salt; Peptide 1 high salt.
E. Peptide 3 low salt; Peptide 1 medium salt; Peptide 2 high salt.
F. Peptide 3 low salt; Peptide 2 medium salt; Peptide 1 high salt.
the commonly used ion exchange DEAE (Diethyl-amino-ethyl) at neutral pH. You want to separate the following mixture of 3 polypeptides on a DEAE column: Peptide 1: MSRNVIKDCKTERYF Peptide 2: MEQNVNQDCKTERYD Peptide 3: MKDHSELDCEHEDHF What will be the order in which these peptides elute from the column as you progressively increase the concentration of KCl in pH 7.0 buffer applied to the column. To keep it simple, we'll just refer to low salt; medium salt; high salt eluted fractions.
A. Peptide 1 low salt; Peptide 2 medium salt; Peptide 3 high salt.
B. Peptide 1 low salt; Peptide 3 medium salt; Peptide 2 high salt.
C. Peptide 2 low salt; Peptide 1 medium salt; Peptide 3 high salt.
D. Peptide 2 low salt; Peptide 3 medium salt; Peptide 1 high salt.
E. Peptide 3 low salt; Peptide 1 medium salt; Peptide 2 high salt.
F. Peptide 3 low salt; Peptide 2 medium salt; Peptide 1 high salt.
