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What are the differences between cDNA vs. Genomic DNA vs. mitochondrial DNA vs plasmid DNA?

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Sixta Kovacek
Sixta KovacekLv2
28 Sep 2019
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A research group has sequenced the cDNA and genomic DNA from a particular gene. The cDNA is derived from mRNA, so it does not contain introns. Here are the DNA sequences. cDNA: 5ʹ–ATTGCATCCAGCGTATACTATCTCGGGCCCAATTAAT - GCCAGCGGCCAGACTATCACCCAACTCGGTTACCTACTAG - TATATCCCATATACTAGCATATATTTTACCCATAATTTGTGTGT - GGGTATACAGTATAATCATATA–3ʹ Genomic DNA (contains one intron): 5ʹ-ATTGCATCCAGCGTATACTATCTCGGGCCCAAT - TAATGCCAGCGGCCAGACTATCACCCAACTCG - GCCCACCCCCCAGGTTTACACAGTCATACCATACA TACAAAAATCGCAGTTACTTATCCCAAAAAAACCTAG - ATACCCCACATACTATTAACTCTTTCTTTCTAGGTTACCTAC - TAGTATATCCCATATACTAGCATATATTTTACCCATAATTTGT - GTGTGGGTATACAGTATAATCATATA–3ʹ Indicate where the intron is located. Does the intron contain the normal consensus splice site sequences based on those described in Figure 14.19? Underline the splice site sequences, and indicate whether or not they fit the consensus sequence.

Question on "Plasmid conformations and mobility on agarose gel vs. oxidative stress"

Extra resource on this subject: http://www3.interscience.wiley.com/cgi-bin/fulltext/113449269/PDFSTART

a) Summarize plasmid conformations and mobility on agarose gel vs. oxidative stress.

b) How could you assess if a plasmid was completely digested by a restriction enzyme or not by exclusively considering the results from agarose gel electrophoresis?

c) Could you estimate the size of a supercoiled plasmid using the MassRuler Express Forward DNA ladder?

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