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A research group has sequenced the cDNA and genomic DNA from a particular gene. The cDNA is derived from mRNA, so it does not contain introns. Here are the DNA sequences. cDNA: 5ʹ–ATTGCATCCAGCGTATACTATCTCGGGCCCAATTAAT - GCCAGCGGCCAGACTATCACCCAACTCGGTTACCTACTAG - TATATCCCATATACTAGCATATATTTTACCCATAATTTGTGTGT - GGGTATACAGTATAATCATATA–3ʹ Genomic DNA (contains one intron): 5ʹ-ATTGCATCCAGCGTATACTATCTCGGGCCCAAT - TAATGCCAGCGGCCAGACTATCACCCAACTCG - GCCCACCCCCCAGGTTTACACAGTCATACCATACA TACAAAAATCGCAGTTACTTATCCCAAAAAAACCTAG - ATACCCCACATACTATTAACTCTTTCTTTCTAGGTTACCTAC - TAGTATATCCCATATACTAGCATATATTTTACCCATAATTTGT - GTGTGGGTATACAGTATAATCATATA–3ʹ Indicate where the intron is located. Does the intron contain the normal consensus splice site sequences based on those described in Figure 14.19? Underline the splice site sequences, and indicate whether or not they fit the consensus sequence.

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Keith Leannon
Keith LeannonLv2
29 Sep 2019

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Related questions

1 DNA sequences found in introns provide [A] amino acid sequence information.

[B] regulatory information.
[C] no known useful information.

[D] structure for the gene.
[E] alternative DNA splicing possibilities.

3. The TATA box is a(n)
[A] sequence close to the promoter region of many genes.
[B] square-shaped sequence.
[C] enhancer consensus sequence.
[D] activator sequence necessary for proper translation.
[E] None of the above

5. Regarding genes and gene expression, which of the following is not a difference between prokaryotes and eukaryotes
[A] Eukaryotic DNA does not contain a TATA box close to the initiation site as does prokaryotic DNA.

[B] In eukaryotes, RNA polymerase requires

'transcription factors'or initiation.

[C] Prokaryotic genes are often grouped together in

operons.

[D] In eukaryotes, the regulation of several genes at once requires common control elements in each of the genes.
[E] There is a diversity of eukaryotic polymerases that is reflected in the diversity of eukaryotic promoters.

10) In eukaryotic cells, a repressor

[A] is made of DNA.
[B] binds to the enhancer region to block transcription. [

C] is located both upstream and downstream from the promoter.
[D] binds to the operator to block RNA polymerase.

[E] binds to a silencer to reduce transcription rates.

Coordinated regulation of genes in eukaryotic cells [A] is the result of positioning the same regulatory sequence in front of each gene.
[B] results from all of those genes being under the control of one promoter.

[C] occurs because related genes all have the same

operons.
[D] occurs because enhancers cause DNA to bend.

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