You are interested in cell size regulation and discover thatsignaling through a GPCR is important in controlling cell size inrat white blood cells. The G protein downstream of this receptoractivates adenylyl cyclase, which ultimately leads to theactivation of PKA. You find that cells that lack the GPCR are 15%smaller than normal cells, whereas cells that express a mutant,constitutively activated version of PKA are 15% larger than normalcells. Furthermore, the normal blood cells become smaller whentreated with cholera toxin, which has been shown to inhibit certainsubclasses of the α subunit of the G protein. Given these results,explain what you predict would happen to the cel's size (bigger,smaller, or no change) if cells were treated in the followingfashion.
You add pertussis toxin.
-
You add a drug that increases the activity of cyclic AMP
phosphodiesterase.
You add a drug that inhibits adenylyl cyclase.
please explain why also.
You are interested in cell size regulation and discover thatsignaling through a GPCR is important in controlling cell size inrat white blood cells. The G protein downstream of this receptoractivates adenylyl cyclase, which ultimately leads to theactivation of PKA. You find that cells that lack the GPCR are 15%smaller than normal cells, whereas cells that express a mutant,constitutively activated version of PKA are 15% larger than normalcells. Furthermore, the normal blood cells become smaller whentreated with cholera toxin, which has been shown to inhibit certainsubclasses of the α subunit of the G protein. Given these results,explain what you predict would happen to the cel's size (bigger,smaller, or no change) if cells were treated in the followingfashion.
You add pertussis toxin.
-
You add a drug that increases the activity of cyclic AMP
phosphodiesterase.
You add a drug that inhibits adenylyl cyclase.
please explain why also.
